Home LiteratureArticle Details
PMID: 3684591 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of the telomeric sequence of the acellular slime molds Didymium iridis and Physarum polycephalum.

Nucleic acids research ·Vol. 15 ·No. 22 ·1987-11-25 ·Pages 9143-52

Forney J, Henderson ER, Blackburn EH

Abstract

We have determined the telomeric DNA sequence of the acellular slime molds Didymium iridis and Physarum polycephalum. In both organisms the telomeres consist of tandem repeats of the hexamer 5'(TTAGGG)3'. This sequence was determined by cloning and sequencing the telomeric fragment of the linear extrachromosomal ribosomal DNA from Didymium, as well as direct end labeling and sequencing the rDNA from both organisms. Interestingly, this sequence is identical to the telomeric DNA sequence of the flagellated protozoan Trypanosoma brucei, and suggests that despite the diversity of telomeric sequences previously determined in lower eukaryotes, the necessity to create functional telomeres has led to constraints on these sequences.

MeSH Terms
Base Sequence Chromosomes/analysis Cloning, Molecular Molecular Sequence Data Myxomycetes/genetics Nucleic Acid Hybridization Physarum/genetics Repetitive Sequences, Nucleic Acid Species Specificity
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Forney J
Department of Molecular Biology, University of California, Berkeley 94720.
Henderson E R
Blackburn E H
References (28)
28 references, click to expand
  1. New concepts of kingdoms or organisms. Evolutionary relations are better represented by new classifications than by the traditional two kingdoms.
    Science. 1969 Jan 10;163(3863):150-60 PMID: 5762760
  2. Dynamics of telomere length variation in Tetrahymena thermophila.
    Cell. 1987 Jul 31;50(3):477-83 PMID: 3607876
  3. Organisation of genes for ribosomal RNA in Physarum polycephalum.
    Eur J Biochem. 1976 Sep 15;68(2):541-9 PMID: 987907
  4. Structure of ribosomal DNA in Physarum polycephalum.
    J Mol Biol. 1976 Sep 25;106(3):567-87 PMID: 988186
  5. Serine proteases: structure and mechanism of catalysis.
    Annu Rev Biochem. 1977;46:331-58 PMID: 332063
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena.
    J Mol Biol. 1978 Mar 25;120(1):33-53 PMID: 642006
  8. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  9. A family of inverted repeat sequences and specific single-strand gaps at the termini of the Physarum rDNA palindrome.
    Cell. 1980 Dec;22(3):875-86 PMID: 7460016
  10. Sequence-specific fragmentation of macronuclear DNA in a holotrichous ciliate.
    Cell. 1981 May;24(2):313-20 PMID: 6786754
  11. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3015-9 PMID: 6265931
  12. An irregular satellite sequence is found at the termini of the linear extrachromosomal rDNA in Dictyostelium discoideum.
    Cell. 1981 Nov;26(3 Pt 1):411-9 PMID: 6276016
  13. Incompletely base-paired flip-flop terminal loops link the two DNA strands of the vaccinia virus genome into one uninterrupted polynucleotide chain.
    Cell. 1982 Feb;28(2):315-24 PMID: 7060133
  14. Repeated hexanucleotide C-C-C-C-A-A is present near free ends of macronuclear DNA of Tetrahymena.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7436-9 PMID: 6950385
  15. Cloning yeast telomeres on linear plasmid vectors.
    Cell. 1982 May;29(1):245-55 PMID: 6286143
  16. Growth of chromosome ends in multiplying trypanosomes.
    Nature. 1983 Jun 16-22;303(5918):592-7 PMID: 6304531
  17. The replication of adenovirus DNA with purified proteins.
    Cell. 1983 Nov;35(1):7-9 PMID: 6313228
  18. Telomeric properties of C4A4-homologous sequences in micronuclear DNA of Oxytricha fallax.
    Cell. 1984 Jan;36(1):171-7 PMID: 6319006
  19. Identification of a telomeric DNA sequence in Trypanosoma brucei.
    Cell. 1984 Feb;36(2):447-57 PMID: 6319025
  20. Structure of the growing telomeres of Trypanosomes.
    Cell. 1984 Feb;36(2):459-68 PMID: 6319026
  21. Elaboration of telomeres in yeast: recognition and modification of termini from Oxytricha macronuclear DNA.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1475-9 PMID: 6324194
  22. DNA sequences of telomeres maintained in yeast.
    Nature. 1984 Jul 12-18;310(5973):154-7 PMID: 6330571
  23. Identification of a telomeric DNA sequence in Plasmodium berghei.
    EMBO J. 1985 Nov;4(11):2991-5 PMID: 2998771
  24. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts.
    Cell. 1985 Dec;43(2 Pt 1):405-13 PMID: 3907856
  25. Evolutionary diversity of eukaryotic small-subunit rRNA genes.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1383-7 PMID: 2419907
  26. Replication and resolution of cloned poxvirus telomeres in vivo generates linear minichromosomes with intact viral hairpin termini.
    J Virol. 1986 Aug;59(2):249-59 PMID: 3016294
  27. Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.
    Nucleic Acids Res. 1987 Feb 25;15(4):1717-28 PMID: 3029725
  28. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.22.9143
Published
1987-11-25
Pages
9143-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306458
Subset
IM
Grants
NIGMS NIH HHS · GM26259 · United States
External Links
PubMed source DOI: 10.1093/nar/15.22.9143 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2