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PMID: 3680370 Published · ppublish English Journal Article

Polarized secretion of a platelet-derived growth factor-like chemotactic factor by endothelial cells in vitro.

The Journal of cell biology ·Vol. 105 ·No. 5 ·1987-11-00 ·Pages 2037-41

Zerwes HG, Risau W

Abstract

Cultured bovine aortic endothelial cells secrete a potent migration-stimulating factor for vascular smooth muscle cells (SMCs) and adventitial fibroblasts. Vascular pericytes are 20-fold less responsive, and endothelial cells themselves do not respond at all. Checkerboard analysis of SMC migration in a micro-chemotaxis chamber assay shows that the factor is chemotactic. Chemotactic activity for SMCs and adventitial fibroblasts is specifically inhibited by antibodies against platelet-derived growth factor. Endothelial cells cultured on nitrocellulose filters secrete the platelet-derived growth factor-like factor almost exclusively into the basal compartment. We suggest that this factor plays an important role in the recruitment of vascular wall cells during the morphogenesis of blood vessels and pathological conditions, such as atherosclerosis.

MeSH Terms
Animals Aorta, Thoracic/physiology Capillaries/physiology Cattle Cells, Cultured Chemotactic Factors/metabolism,physiology Chemotaxis Endothelium, Vascular/physiology Muscle, Smooth, Vascular/physiology Platelet-Derived Growth Factor/metabolism,physiology
Chemicals
Chemotactic Factors Platelet-Derived Growth Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zerwes H G
Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Federal Republic of Germany.
Risau W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.5.2037
Published
1987-11-00
Pages
2037-41
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114851
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.105.5.2037 PMC Full Text View journal details
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