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PMID: 3673509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Selective kainic acid lesions in cultured explants of rat hippocampus.

Acta neuropathologica ·Vol. 74 ·No. 2 ·1987-00-00 ·Pages 183-90

Rimvall K, Keller F, Waser PG

Abstract

The influence of the excitotoxin kainic acid (KA) on cultivated explants of rat hippocampus was investigated. Addition of 3 microM KA to the culture medium over 24-48 h induced a destruction of the pyramidal cells in the CA3 region, whereas the CA1 pyramidal cells and the granule cells were left undamaged. Higher concentrations (10-100 microM) of KA destroyed also the latter cell groups. The selectivity of the KA lesion at 3 microM was further indicated by the fact that the acetylcholinesterase-positive neurons in the hippocampus were not destroyed through KA administration and that the stereoisomer dihydrokainic acid was ineffective in inducing lesions. Application of tetrodotoxin did not protect the CA3 pyramidal cells from KA lesion, whereas gamma-glutamylaminomethylsulphonic acid (GAMS) only offered a very small, statistically not significant, protection. Baclofen protected the cultures slightly from KA lesions but not when added together with GAMS. Possible mechanisms responsible for the KA lesions in these cultures are discussed.

MeSH Terms
Acetylcholinesterase/metabolism Animals Culture Techniques Hippocampus/drug effects,enzymology,pathology Histocytochemistry Kainic Acid/pharmacology Osmolar Concentration Rats Time Factors
Chemicals
Acetylcholinesterase Kainic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rimvall K
Institute of Pharmacology, University of Zurich, Switzerland.
Keller F
Waser P G
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40 references, click to expand
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Article Info
Journal
Acta neuropathologica
Abbr.
Acta Neuropathol
ISSN
0001-6322
DOI
10.1007/BF00692850
Published
1987-00-00
Pages
183-90
Language
English
Region
Germany
NLM ID
0412041
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00692850 View journal details
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