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PMID: 366382 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations.

Molecular & general genetics : MGG ·Vol. 167 ·No. 2 ·1978-11-29 ·Pages 129-37

Kreuzer KN, McEntee K, Geballe AP, Cozzarelli NR

Abstract

Defective lambda transducing phages for the nalA region of the Escherichia coli chromosome were isolated from a lysogen in which lambda is inserted in the nearby glpT gene. The three classes of transducing phages designated lambdanrdA, lambdaubiG, and lambdadnalA contained bacterial DNA extending from glpT through nrdA, ubiG, and nalA, respectively. The bacterial genes are in the left arm of the lambda chromosome. Of the eleven polypeptides coded by lambdadnalA that were resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate only one was not also specified by lambdadubiG. This 105,000 dalton polypeptide is the nalA gene product. The electorphoretic mobility and isoelectric point of this protein were unaffected by a nalA mutation (nalA48) that confers nalidixic acid resistance. Temperature-sensitive and amber mutations in the nalA gene were isolated using a lambdadnalA48 lysogen which is heterodiploid for nalA. The conditional lethality of these mutations proves that nalA is an essential locus.

MeSH Terms
Bacterial Proteins/biosynthesis Coliphages/genetics Defective Viruses/genetics Drug Resistance, Microbial Escherichia coli/genetics Genes, Lethal Mutation Nalidixic Acid/pharmacology Transduction, Genetic
Chemicals
Bacterial Proteins Nalidixic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kreuzer K N
McEntee K
Geballe A P
Cozzarelli N R
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38 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00266906
Published
1978-11-29
Pages
129-37
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00266906
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