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PMID: 3663100 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Measurement of allantoin and uric acid in human body fluids. A potential index of free-radical reactions in vivo?

The Biochemical journal ·Vol. 243 ·No. 3 ·1987-05-01 ·Pages 803-8

Grootveld M, Halliwell B

Abstract

Free-radical attack upon uric acid generates allantoin [Ames, Cathcart, Schwiers & Hochstein (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 6858-6862]. Methods are described for the accurate measurement of uric acid and allantoin in human body fluids. The concentrations of uric acid and allantoin in human serum and synovial fluid are reported. It is suggested that measurement of changes in allantoin concentration may be a useful index of free-radical reactions taking place in vivo.

MeSH Terms
Allantoin/metabolism Body Fluids/metabolism Chromatography, High Pressure Liquid Electrochemistry Free Radicals Glyoxylates/isolation & purification Humans In Vitro Techniques Spectrophotometry, Ultraviolet Uric Acid/metabolism
Chemicals
Free Radicals Glyoxylates Uric Acid glyoxylate 2,4-dinitrophenylhydrazone Allantoin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grootveld M
Department of Biochemistry, King's College (KQC), Strand Campus, London, U.K.
Halliwell B
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20 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2430803
Published
1987-05-01
Pages
803-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147928
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2430803 PMC Full Text View journal details
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