The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties.

Biochimica et biophysica acta ·Vol. 567 ·No. 2 ·1979-04-12 ·Pages 315-24

Schink B, Schlegel HG

Abstract

The membrane-bound hydrogenase of Alcaligenes eutrophus was solubilized from washed membranes of autotrophically grown cells. The enzyme consists of two types of subunits and is an iron-sulfur protein. A flavin compound was not detected. The enzyme reacts only with few artificial electron acceptors.

MeSH Terms

Alcaligenes/enzymology Cell Membrane/enzymology Deoxycholic Acid Hydrogen Hydrogen-Ion Concentration Iron-Sulfur Proteins Methylene Blue/metabolism Molecular Weight Oxidoreductases/isolation & purification,metabolism Phosphatidylcholines/pharmacology Polyethylene Glycols Solubility Substrate Specificity

Chemicals

Iron-Sulfur Proteins Phosphatidylcholines Deoxycholic Acid Polyethylene Glycols Hydrogen Oxidoreductases Methylene Blue

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Schink B

Schlegel H G

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0005-2744(79)90117-7

Published

1979-04-12

Pages

315-24

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/0005-2744(79)90117-7

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The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties.

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