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PMID: 36114582 Published · epublish English Journal Article Video-Audio Media Research Support, Non-U.S. Gov't

RhoB affects colitis through modulating cell signaling and intestinal microbiome.

Microbiome ·Vol. 10 ·No. 1 ·2022-00-16 ·Pages 149

Yang J, Pei G, Sun X, Xiao Y, Miao C, Zhou L, Wang B, Yang L, Yu M, Zhang ZS, Keller ET, Yao Z, Wang Q

Abstract

The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed. RhoB regulates multiple cell functions; however, its role in colitis is unexplored. Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis. Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation. Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway. Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed. Taken together, a higher level of RhoB is associated with UC, which also contributes to UC development through modulating cell signaling and altering intestinal bacterial composition and metabolites. These observations suggest that RhoB has potential as a biomarker and a treatment target for UC. Video Abstract.

Keywords
Inflammatory bowel diseases Microbiome RhoB SCFAs
MeSH Terms
Animals Biomarkers Colitis/chemically induced,pathology Colitis, Ulcerative/metabolism,microbiology,pathology Dextran Sulfate Gastrointestinal Microbiome Humans Mice Signal Transduction p38 Mitogen-Activated Protein Kinases/metabolism rhoB GTP-Binding Protein/metabolism
Chemicals
Biomarkers Dextran Sulfate p38 Mitogen-Activated Protein Kinases rhoB GTP-Binding Protein
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Yang Jianming
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China.
Pei Geng
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China.
Sun Xuan
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China.
Xiao Yawen
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China.
Miao Chunhui
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China.
Zhou Lu
Department of Gastroenterology and Hepatology, Tianjin Medical University general hospital, Tianjin Medical University, Tianjin, 300070, China.
Wang Bangmao
Department of Gastroenterology and Hepatology, Tianjin Medical University general hospital, Tianjin Medical University, Tianjin, 300070, China.
Yang Liu
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China.
Yu Mingyu
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China.
Zhang Zhi-Song
State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Collaborative Innovation Center for Biotherapy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300350, China.
Keller Evan T
Department of Urology, Biointerfaces Institute, University of Michigan, Ann Arbor, MI, USA.
Yao Zhi
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China. | The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, Tianjin, 300070, China.
Wang Quan
Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China. wangquan@tmu.edu.cn. | The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, Tianjin, 300070, China. wangquan@tmu.edu.cn.
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Article Info
Journal
Microbiome
Abbr.
Microbiome
ISSN
2049-2618
DOI
10.1186/s40168-022-01347-3
Email
wangquan@tmu.edu.cn
Published
2022-00-16
Epub
2022-00-16
Pages
149
Language
English
Region
England
NLM ID
101615147
PMCID
PMC9482252
Subset
IM
External Links
PubMed source DOI: 10.1186/s40168-022-01347-3 PMC Full Text View journal details
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