Maintenance of membrane phospholipid asymmetry. Lipid-cytoskeletal interactions or lipid pump?

Two models for the mechanism of maintenance of lipid asymmetry in erythrocytes are considered: binding of internal lipids to cytoskeletal proteins, and pumping of internal lipids from the outside to the inside of the cell. Analysis of the kinetics of lipid internalization suggests that the first model is more likely, and that the apparent pumping of lipids represents the activity of an ATP-dependent lipid flip/flop catalyst.