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PMID: 3608984 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The receptor-binding and membrane-fusion properties of influenza virus variants selected using anti-haemagglutinin monoclonal antibodies.

The EMBO journal ·Vol. 6 ·No. 5 ·1987-05-00 ·Pages 1459-65

Daniels PS, Jeffries S, Yates P, Schild GC, Rogers GN, Paulson JC, Wharton SA, Douglas AR, Skehel JJ, Wiley DC

Abstract

A monoclonal antibody raised against X-31 influenza virus reacted with the majority of natural H3N2 viruses isolated between 1968 and 1982. A number of variants of X-31 and of a receptor-binding mutant of X-31 were selected by the antibody during virus replication in eggs and MDCK cells. Antibody-binding assays indicated that the viruses selected were not antigenic variants and analyses using derivatized erythrocytes showed that their receptor-binding properties differed from those of the parent viruses. The amino acid substitutions in the variants were all located in the vicinity of the receptor-binding site and the structural consequences are discussed in relation to the three-dimensional structure of X-31 HA. In addition all of the variants fused membranes at higher pH than wild-type virus indicating that structural modifications in the distal globular region of HA influence the low pH-induced conformational change required for membrane fusion.

MeSH Terms
Animals Antibodies, Monoclonal Genetic Variation Hemagglutination Inhibition Tests Hemagglutinins, Viral/genetics Influenza A virus/genetics,immunology,physiology Mice Mice, Inbred BALB C Mutation Receptors, Virus/physiology Species Specificity
Chemicals
Antibodies, Monoclonal Hemagglutinins, Viral Receptors, Virus
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Daniels P S
Jeffries S
Yates P
Schild G C
Rogers G N
Paulson J C
Wharton S A
Douglas A R
Skehel J J
Wiley D C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-05-00
Pages
1459-65
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553952
Subset
IM
Grants
NIAID NIH HHS · AI 13654 · United States
NIAID NIH HHS · AI20591 · United States
External Links
PubMed source PMC Full Text View journal details
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