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PMID: 360041 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic analysis of two genes, dnaJ and dnaK, necessary for Escherichia coli and bacteriophage lambda DNA replication.

Molecular & general genetics : MGG ·Vol. 164 ·No. 1 ·1978-08-04 ·Pages 9-14

Yochem J, Uchida H, Sunshine M, Saito H, Georgopoulos CP, Feiss M

Abstract

We show that a collection of 93 E. coli mutations which map between thr and leu and which block phage lambda DNA replication define two closely linked cistrons. Work published in the accompanying paper shows that these mutations also affect host DNA replication, so we designate them dnaJ and dnaK; the gene order is thr--dnaK--dnaJ--leu. Demonstration of two cistrons was possible with the isolation of lambda transducing phages carrying one or the other or both of the dna genes. These phages were employed in phage vs bacterial complementation studies which unambiguously show that dnaK and dnaJ are different cistrons.

MeSH Terms

Coliphages/genetics DNA Replication DNA, Bacterial/metabolism Escherichia coli/genetics Genes Mutation Terminology as Topic Virus Replication

Chemicals

DNA, Bacterial

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Yochem J

Uchida H

Sunshine M

Saito H

Georgopoulos C P

Feiss M

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267593

Published

1978-08-04

Pages

9-14

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267593

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Genetic analysis of two genes, dnaJ and dnaK, necessary for Escherichia coli and bacteriophage lambda DNA replication.

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