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PMID: 3595558 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DNA-mediated transformation of the basidiomycete Coprinus cinereus.

The EMBO journal ·Vol. 6 ·No. 4 ·1987-04-00 ·Pages 835-40

Binninger DM, Skrzynia C, Pukkila PJ, Casselton LA

Abstract

We have developed a simple and efficient transformation system for the agaric fungus, Coprinus cinereus. Protoplasts were prepared from asexual spores that harbor one or two mutations in the structural gene for tryptophan synthetase. The protoplasts can be stably transformed using the cloned Coprinus gene at a frequency of 1 in 10(4) viable protoplasts. A variety of molecular events accompanies the formation of stable transformants, including insertion of the transforming DNA at the homologous locus. The transforming DNA is stable through cell division, mating, fruiting body formation, and meiosis.

MeSH Terms
Agaricales/genetics Cloning, Molecular Coprinus/enzymology,genetics DNA, Recombinant/metabolism Genes Genes, Fungal Mutation Tryptophan Synthase/genetics
Chemicals
DNA, Recombinant Tryptophan Synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Binninger D M
Skrzynia C
Pukkila P J
Casselton L A
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28 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-04-00
Pages
835-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553472
Subset
IM
Grants
NIGMS NIH HHS · T32-GM07092 · United States
External Links
PubMed source PMC Full Text View journal details
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