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PMID: 3593218 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Adrenergic regulation of formation of inositol phosphates in rat submandibular acini.

The Biochemical journal ·Vol. 241 ·No. 3 ·1987-02-01 ·Pages 705-9

Doughney C, Dormer RL, McPherson MA

Abstract

Formation of inositol phosphates in response to adrenergic secretagogues was studied in rat submandibular acini labelled with myo-[2-3H]inositol. Noradrenaline rapidly (within 5 s) increased radioactivity incorporated into inositol 1,4,5-trisphosphate and inositol tetrakisphosphate, with less rapid (within 1 min) increases in inositol 1,3,4-trisphosphate being observed. Inositol polyphosphate formation was less sensitive to noradrenaline than was stimulation of mucin secretion and was mediated by stimulation of alpha- but not beta-adrenergic receptors. The beta-adrenergic agonist isoproterenol, which is a potent stimulator of mucin secretion [McPherson & Dormer (1984) Biochem. J. 224, 473-481] did not increase formation of inositol mono-, bis- or polyphosphates during a 15 min incubation. The results suggest that inositol phosphates do not mediate beta-adrenergic stimulation of mucin secretion in rat submandibular acini. In addition, rat submandibular acinar cells contain a Ca2+ pool which can be mobilized by isoproterenol [McPherson & Dormer (1984) Biochem. J. 224, 473-481], without involvement of inositol polyphosphates as second messengers.

MeSH Terms

Animals Chromatography, High Pressure Liquid Dose-Response Relationship, Drug In Vitro Techniques Inositol Phosphates/metabolism Isoproterenol/pharmacology Male Norepinephrine/pharmacology Phentolamine/pharmacology Propranolol/pharmacology Rats Rats, Inbred Strains Submandibular Gland/drug effects,metabolism Sugar Phosphates/metabolism Time Factors

Chemicals

Inositol Phosphates Sugar Phosphates Propranolol Isoproterenol Norepinephrine Phentolamine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Doughney C

Dormer R L

McPherson M A

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23 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2410705

Published

1987-02-01

Pages

705-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1147621

Subset

External Links

PubMed source DOI: 10.1042/bj2410705 PMC Full Text View journal details

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Adrenergic regulation of formation of inositol phosphates in rat submandibular acini.

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