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PMID: 3588310 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells.

Nucleic acids research ·Vol. 15 ·No. 9 ·1987-05-11 ·Pages 3773-86

Westley BR, May FE

Abstract

A cDNA library was constructed from the mRNA of the ZR-75 oestrogen responsive human breast cancer cell line and screened for oestrogen regulated mRNA sequences. Of the recombinants isolated, 30 contained cDNA that corresponded to a single mRNA species of 2.1 kb that was induced between 10 and 15 fold by oestradiol treatment. The sequence of the entire open reading frame and 3' non-coding region of the mRNA was determined and shown to encode the aspartyl protease cathepsin D. The induction of cathepsin D mRNA is specific for oestrogen and is maximal at 3 X 10(-10) M. Cathepsin D mRNA levels were increased by oestrogen in 3 oestrogen responsive breast cancer cell lines. Cathepsin D mRNA was expressed but not regulated in an oestrogen receptor negative breast tumour cell line, BT 20, and in 2 other malignant cell lines, Hela and A431.

MeSH Terms
Base Sequence Breast Neoplasms/analysis,pathology Cathepsin D/biosynthesis,genetics Cells, Cultured DNA/analysis Enzyme Induction/drug effects Estradiol/pharmacology Neoplasm Proteins/biosynthesis,genetics Neoplasms, Hormone-Dependent/analysis,pathology RNA, Messenger/biosynthesis,isolation & purification RNA, Neoplasm/biosynthesis,isolation & purification
Chemicals
Neoplasm Proteins RNA, Messenger RNA, Neoplasm Estradiol DNA Cathepsin D
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Westley B R
May F E
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.9.3773
Published
1987-05-11
Pages
3773-86
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340781
Subset
IM
Databases
GENBANK
X05344
External Links
PubMed source DOI: 10.1093/nar/15.9.3773 PMC Full Text View journal details
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