Home LiteratureArticle Details
PMID: 3584077 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Molecular cloning and biological characterization of the recA gene from Pseudomonas syringae.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2906-10

Hickman MJ, Orser CS, Willis DK, Lindow SE, Panopoulos NJ

Abstract

We have identified a recombinant plasmid, pCUV8, from a cosmid library of Pseudomonas syringae genomic DNA which contains a functional analog of the Escherichia coli recA gene. The plasmid was initially identified by its ability to restore UV resistance to E. coli HB101. Quantitative analysis demonstrated that it restored both recombination proficiency and UV resistance to an E. coli recA deletion mutant. By these criteria, pCUV8 appears to contain the P. syringae recA gene. Several pathogenic and epiphytic strains of P. syringae, but not E. coli, showed sequence homology to pCUV8 under normal stringency.

MeSH Terms
Cloning, Molecular DNA, Bacterial/genetics Genes, Bacterial Pseudomonas/genetics Rec A Recombinases/genetics Recombination, Genetic
Chemicals
DNA, Bacterial Rec A Recombinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hickman M J
Orser C S
Willis D K
Lindow S E
Panopoulos N J
References (24)
24 references, click to expand
  1. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.
    Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 PMID: 4873517
  2. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  3. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  4. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  5. Recombination deficient mutants of E. coli and other bacteria.
    Annu Rev Genet. 1973;7:67-86 PMID: 4205905
  6. In vivo studies of temperature-sensitive recB and recC mutants.
    J Bacteriol. 1974 Dec;120(3):1213-8 PMID: 4612007
  7. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  8. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  9. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  10. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  11. Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli K-12 chromosome.
    Genetics. 1979 Oct;93(2):321-43 PMID: 395024
  12. Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.
    Mol Gen Genet. 1981;183(3):497-504 PMID: 6278241
  13. The SOS regulatory system of Escherichia coli.
    Cell. 1982 May;29(1):11-22 PMID: 7049397
  14. Molecular mechanisms in genetic recombination.
    Annu Rev Biochem. 1982;51:727-61 PMID: 6287923
  15. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants.
    Gene. 1982 Jun;18(3):289-96 PMID: 6290332
  16. Isolation and characterization of the recA gene of Rhizobium meliloti.
    J Bacteriol. 1983 Jul;155(1):311-6 PMID: 6305915
  17. Tn5-induced mutations affecting virulence factors of Bordetella pertussis.
    Infect Immun. 1983 Oct;42(1):33-41 PMID: 6311749
  18. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.
    Microbiol Rev. 1984 Mar;48(1):60-93 PMID: 6371470
  19. Cloning and characterization of recA genes froM Proteus vulgaris, Erwinia carotovora, Shigella flexneri, and Escherichia coli B/r.
    J Bacteriol. 1984 Oct;160(1):153-60 PMID: 6090408
  20. Cloning and expression of bacterial ice nucleation genes in Escherichia coli.
    J Bacteriol. 1985 Oct;164(1):359-66 PMID: 3900043
  21. recA is required in the induction of pectin lyase and carotovoricin in Erwinia carotovora subsp. carotovora.
    J Bacteriol. 1985 Oct;164(1):390-6 PMID: 4044526
  22. Two simple media for the demonstration of pyocyanin and fluorescin.
    J Lab Clin Med. 1954 Aug;44(2):301-7 PMID: 13184240
  23. Genetic analysis of a "double male" strain of Escherichia coli K-12.
    Genetics. 1963 Jan;48:105-20 PMID: 14021492
  24. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.
    Genetics. 1964 Feb;49:237-46 PMID: 14124942
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.6.2906-2910.1987
Published
1987-06-00
Pages
2906-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212212
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.6.2906-2910.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2