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PMID: 358190 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Processing of the 5' end of Escherichia coli 16S ribosomal RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 8 ·1978-08-00 ·Pages 3598-602

Dahlberg AE, Dahlberg JE, Lund E, Tokimatsu H, Rabson AB, Calvert PC, Reynolds F, Zahalak M

Abstract

We have isolated and partially characterized an endonuclease involved in processing the 5' end of 16S rRNA of Escherichia coli. A mutant strain that is deficient in this enzyme accumulates a new precursor of 16S rRNA, named 16.3S rRNA. This rRNA has the 3' end of mature 16S rRNA but is about 60 nucleotides longer at the 5' end. In vitro, the enzyme preparation cleaves an RNA fragment of about 60 nucleotides from the 5' end of 16.3S rRNA in 30S ribosomal subunits, yielding the mature 5' end of 16S rRNA. In the mutant strain the 16.3S rRNA is associated with a full complement of 21 ribosomal proteins in 30S subunits. These particles, which comprise 50% of the total 30S subunits, are present on polyribosomes.

MeSH Terms
Escherichia coli/genetics Nucleic Acid Precursors/metabolism RNA, Bacterial/metabolism RNA, Ribosomal/metabolism Ribonucleases/isolation & purification,metabolism Ribosomal Proteins Transcription, Genetic
Chemicals
Nucleic Acid Precursors RNA, Bacterial RNA, Ribosomal Ribosomal Proteins Ribonucleases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Dahlberg A E
Dahlberg J E
Lund E
Tokimatsu H
Rabson A B
Calvert P C
Reynolds F
Zahalak M
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.8.3598
Published
1978-08-00
Pages
3598-602
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392832
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.8.3598 PMC Full Text View journal details
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