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PMID: 3570467 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Defensins mediate the microbicidal activity of human neutrophil granule extract against Acinetobacter calcoaceticus.

Infection and immunity ·Vol. 55 ·No. 6 ·1987-06-00 ·Pages 1365-8

Greenwald GI, Ganz T

Abstract

An acid extract of human neutrophil granules was fractionated on a Sephadex G-100 column and tested for microbicidal activity against Acinetobacter calcoaceticus HO-1 as described previously (M.C. Modrzakowski and C. M. Paranavitana, Infect. Immun. 32:668-674, 1981). The low-molecular-weight protein fraction, peak D, accounted for about 30% of the protein and nearly all of the activity of the crude extract against strain HO-1. Peak D protein proved to be a mixture of the three human defensin peptides HNP1, HNP2, and HNP3. Purified defensins reproduced the microbicidal activity of peak D. The data suggest that defensins could play a major role in the killing of A. calcoaceticus by human neutrophils.

MeSH Terms
Acinetobacter/drug effects Acinetobacter Infections/immunology Blood Proteins/isolation & purification,pharmacology Dose-Response Relationship, Drug Humans Neutrophils/physiology alpha-Defensins
Chemicals
Blood Proteins alpha-Defensins human neutrophil peptide 1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Greenwald G I
Ganz T
References (19)
19 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.6.1365-1368.1987
Published
1987-06-00
Pages
1365-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260521
Subset
IM
Grants
NIAID NIH HHS · AI 21931 · United States
NHLBI NIH HHS · HL 35640 · United States
External Links
PubMed source DOI: 10.1128/iai.55.6.1365-1368.1987 PMC Full Text View journal details
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