Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments.

An easy manipulation of the commercial fluorescence microscope allows stereo pairs of pictures to be taken, which when examined with a stereo viewer, give a strong three-dimensional impression. The procedure is described in detail. Its use allows the documentation by immunofluorescence microscopy of the three-dimensional display and organization of microfilament bundles, microtubules and tonofilaments in some well characterized tissue culture cell lines.