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PMID: 3562246 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oligodeoxyribonucleotides containing 1,3-propanediol as nucleoside substitute.

Nucleic acids research ·Vol. 15 ·No. 7 ·1987-04-10 ·Pages 3113-29

Seela F, Kaiser K

Abstract

1,3-Propanediol was protected with one dimethoxytrityl residue and converted into the methoxy- and cyanoethoxyphosphoramidites 2a and 2b, respectively. Solid-phase oligonucleotide synthesis, employing the phosphoramidite 2a resulted in the dodecamers d(CGCGAATTCGCG) (6-9), in which dA or dT residues were replaced by 1,3-propanediol. These oligomers showed a high tendency to form hairpins. Their phosphodiester bonds between the 3'-position of a nucleoside and the propanediol moiety was not cleaved by snake venom phosphodiesterase.

MeSH Terms

Base Sequence Indicators and Reagents Nucleic Acid Denaturation Oligodeoxyribonucleotides/chemical synthesis Propylene Glycols Structure-Activity Relationship Thermodynamics

Chemicals

Indicators and Reagents Oligodeoxyribonucleotides Propylene Glycols 1,3-propanediol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Seela F

Kaiser K

References (12)

12 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/15.7.3113

Published

1987-04-10

Pages

3113-29

Language

English

Region

England

NLM ID

0411011

PMCID

PMC340719

Subset

External Links

PubMed source DOI: 10.1093/nar/15.7.3113 PMC Full Text View journal details

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