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PMID: 356045 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

ATP-induced conformational changes in the restriction endonuclease from Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 7 ·1978-07-00 ·Pages 3099-103

Bickle TA, Brack C, Yuan R

Abstract

ATP induces a conformational change in the Escherichia coli K-12 restriction enzyme that allows it to discriminate between unmodified and modified DNA recognition sequences. This conformational change does not require ATP hydrolysis. However, ATP hydrolysis is a requirement for DNA cleavage.

MeSH Terms
Adenosine Triphosphate/metabolism,pharmacology Binding Sites Coliphages DNA Restriction Enzymes/metabolism DNA, Viral/metabolism Escherichia coli/enzymology Methylation Protein Conformation/drug effects S-Adenosylmethionine/metabolism Substrate Specificity/drug effects
Chemicals
DNA, Viral S-Adenosylmethionine Adenosine Triphosphate DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bickle T A
Brack C
Yuan R
References (11)
11 references, click to expand
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    Proc Natl Acad Sci U S A. 1970 Feb;65(2):357-62 PMID: 4984237
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    Nature. 1968 Mar 23;217(5134):1110-4 PMID: 4868368
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.7.3099
Published
1978-07-00
Pages
3099-103
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392721
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.7.3099 PMC Full Text View journal details
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