Home LiteratureArticle Details
PMID: 3553160 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Modification, processing, and subcellular localization in Escherichia coli of the pCloDF13-encoded bacteriocin release protein fused to the mature portion of beta-lactamase.

Journal of bacteriology ·Vol. 169 ·No. 5 ·1987-05-00 ·Pages 2245-50

Luirink J, Watanabe T, Wu HC, Stegehuis F, de Graaf FK, Oudega B

Abstract

A fusion between the pCloDF13-derived bacteriocin release protein and beta-lactamase was constructed to investigate the subcellular localization and posttranslational modification of the bacteriocin release protein in Escherichia coli. The signal sequence and 25 of the 28 amino acid residues of the mature bacteriocin release protein were fused to the mature portion of beta-lactamase. The hybrid protein (Mr, 31,588) was expressed in minicells and whole cells and possessed full beta-lactamase activity. Immunoblotting of subcellular fractions revealed that the hybrid protein is present in both the cytoplasmic and outer membranes of E. coli. Radioactive labeling experiments in the presence or absence of globomycin showed that the hybrid protein is modified with a diglyceride and fatty acids and is processed by signal peptidase II, as is the murein lipoprotein. The results indicated that the pCloDF13-encoded bacteriocin release protein is a lipoprotein which is associated with both membranes of E. coli cells.

MeSH Terms
Bacterial Proteins/metabolism Bacteriocins/metabolism Cell Compartmentation Cloning, Molecular Diglycerides/metabolism Escherichia coli/metabolism Escherichia coli Proteins Fatty Acids/metabolism Protein Processing, Post-Translational Protein Sorting Signals/metabolism Recombinant Fusion Proteins/metabolism beta-Lactamases/metabolism
Chemicals
Bacterial Proteins Bacteriocins Diglycerides Escherichia coli Proteins Fatty Acids Protein Sorting Signals Recombinant Fusion Proteins cloacin DF13-encoded bacteriocin release protein, E coli beta-Lactamases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Luirink J
Watanabe T
Wu H C
Stegehuis F
de Graaf F K
Oudega B
References (40)
40 references, click to expand
  1. Characterization and nucleotide sequence of a colicin-release gene in the hic region of plasmid ColE3-CA38.
    Gene. 1984 Jul-Aug;29(1-2):175-84 PMID: 6092219
  2. The role of the beta-lactamase signal sequence in the secretion of proteins by Escherichia coli.
    J Biol Chem. 1984 Feb 25;259(4):2149-54 PMID: 6365904
  3. Inhibition of prolipoprotein signal peptidase by globomycin.
    J Biol Chem. 1985 May 25;260(10):5891-4 PMID: 3888977
  4. Lysis protein encoded by plasmid ColA-CA31. Gene sequence and export.
    Mol Gen Genet. 1985;199(1):95-100 PMID: 3889552
  5. Molecular characterisation of the colicin E2 operon and identification of its products.
    Mol Gen Genet. 1985;198(3):465-72 PMID: 3892228
  6. Failure of E. coli K-12 to transport PhoE-LacZ hybrid proteins out of the cytoplasm.
    EMBO J. 1985 Apr;4(4):1041-7 PMID: 2990905
  7. Trimeric structure and localization of the major lipoprotein in the cell surface of Escherichia coli.
    J Biol Chem. 1986 Jul 5;261(19):8953-7 PMID: 3013869
  8. Effects of divalent cations and of phospholipase A activity on excretion of cloacin DF13 and lysis of host cells.
    J Gen Microbiol. 1986 Mar;132(3):825-34 PMID: 3016150
  9. Biogenesis of lipoproteins in bacteria.
    Curr Top Microbiol Immunol. 1986;125:127-57 PMID: 3527576
  10. Production and release of cloacin DF13 and related colicins.
    Curr Top Microbiol Immunol. 1986;125:183-205 PMID: 3527577
  11. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.
    Mol Gen Genet. 1979 May 4;172(2):151-9 PMID: 384144
  12. Uncoupling of synthesis and release of cloacin DF13 and its immunity protein by Escherichia coli.
    Mol Gen Genet. 1987 Jan;206(1):126-32 PMID: 3553860
  13. The ins and outs of colicins. Part I: Production, and translocation across membranes.
    Microbiol Sci. 1984 Oct;1(7):168-75 PMID: 6444117
  14. On the localization of alkaline phosphatase and cyclic phosphodiesterase in Escherichia coli.
    Biochemistry. 1968 Jul;7(7):2554-62 PMID: 4298222
  15. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  16. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  17. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.
    J Bacteriol. 1973 Sep;115(3):717-22 PMID: 4580564
  18. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
    Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
  19. Covalent lipoprotein from the outer membrane of Escherichia coli.
    Biochim Biophys Acta. 1975 Oct 31;415(3):335-77 PMID: 52377
  20. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
    FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
  21. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli.
    Anal Biochem. 1976 Jul;74(1):160-70 PMID: 786067
  22. The transposon Tn1 as a probe for studying ColE1 structure and function.
    Mol Gen Genet. 1977 Mar 7;151(2):151-60 PMID: 327263
  23. Mechanism of action of globomycin.
    J Antibiot (Tokyo). 1978 Nov;31(11):1203-5 PMID: 721715
  24. Factors necessary for the export process of colicin E1 across cytoplasmic membrane of Escherichia coli.
    Eur J Biochem. 1984 Apr 16;140(2):249-55 PMID: 6425060
  25. Studies on the modification and processing of prolipoprotein in Escherichia coli. Effects of structural alterations in prolipoprotein on its maturation in wild type and lpp mutants.
    J Biol Chem. 1984 May 25;259(10):6098-104 PMID: 6373750
  26. Production and excretion of cloacin DF13 by Escherichia coli harboring plasmid CloDF13.
    J Bacteriol. 1979 Nov;140(2):415-23 PMID: 227839
  27. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  28. Comparison of the polypeptide composition of Escherichia coli outer membranes prepared by two methods.
    J Bacteriol. 1980 Oct;144(1):425-7 PMID: 6998960
  29. Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli.
    J Bacteriol. 1981 Apr;146(1):325-30 PMID: 6260742
  30. Organization and expression of genes involved in the production of the K88ab antigen.
    Infect Immun. 1981 Jun;32(3):1155-63 PMID: 7019089
  31. Lipoprotein nature of Bacillus licheniformis membrane penicillinase.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3511-5 PMID: 7022453
  32. Protein H encoded by plasmid Clo DF13 involved in lysis of the bacterial host. I. Localisation of the gene and identification and subcellular localisation of the gene H product.
    Mol Gen Genet. 1981;183(2):318-25 PMID: 6276683
  33. Colicins and other bacteriocins with established modes of action.
    Annu Rev Microbiol. 1982;36:125-44 PMID: 6184011
  34. Translocation of colicin E1 through cytoplasmic membrane of Escherichia coli.
    FEBS Lett. 1982 Dec 27;150(2):465-8 PMID: 6761149
  35. Alternative forms of lethality in mitomycin C-induced bacteria carrying ColE1 plasmids.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):579-83 PMID: 6403939
  36. A genetic approach to the study of mitomycin-induced lysis of Escherichia coli K-12 strains which produce colicin E2.
    Mol Gen Genet. 1983;190(3):366-72 PMID: 6308394
  37. Expression of a gene in a 400-base-pair fragment of colicin plasmid ColE2-P9 is sufficient to cause host cell lysis.
    J Bacteriol. 1983 Oct;156(1):109-14 PMID: 6352670
  38. Biosynthesis of Bacillus licheniformis penicillinase in Escherichia coli and in Bacillus subtilis.
    J Bacteriol. 1983 Nov;156(2):773-7 PMID: 6415038
  39. Plasmid ColE3 specifies a lysis protein.
    J Bacteriol. 1984 Feb;157(2):582-90 PMID: 6319368
  40. Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase.
    EMBO J. 1984 Oct;3(10):2393-7 PMID: 6389120
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.5.2245-2250.1987
Published
1987-05-00
Pages
2245-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212142
Subset
IM
Grants
NIGMS NIH HHS · GM-28811 · United States
External Links
PubMed source DOI: 10.1128/jb.169.5.2245-2250.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2