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PMID: 3550809 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translocation of an unusual cAMP receptor to the nucleus during development of Dictyostelium discoideum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 8 ·1987-04-00 ·Pages 2322-6

Kay CA, Noce T, Tsang AS

Abstract

cAMP has been implicated in the control of the expression of developmental genes in Dictyostelium discoideum. To determine the potential role of cAMP receptors as regulators of gene expression, we have used immunocytochemical and immunoblotting techniques to reveal the subcellular localization of a cAMP binding protein CABP1. Most of the CABP1 antigen in early developing cells is localized near the cell periphery, with a small amount found in the nucleus. The level of CABP1 in the nucleus increases approximately 30-fold during development. Moreover, immunofluorescence studies showed that CABP1 can also be detected on the cell surface. Binding of anti-CABP1 to intact cells followed by reaction with 125I-labeled secondary antibody revealed that the cell-surface CABP1 activity peaks during aggregation and culmination. In addition, several proteins related to CABP1 are found mainly in the nuclear fraction of developing cells. The possible role of these proteins in the regulation of developmental gene activity is discussed.

MeSH Terms
Cell Nucleus/metabolism Dictyostelium/genetics,growth & development Fluorescent Antibody Technique Gene Expression Regulation Genes, Fungal Receptors, Cyclic AMP/metabolism
Chemicals
Receptors, Cyclic AMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kay C A
Noce T
Tsang A S
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.8.2322
Published
1987-04-00
Pages
2322-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304642
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.8.2322 PMC Full Text View journal details
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