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PMID: 3549284 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Both hydrophobic domains of M13 procoat are required to initiate membrane insertion.

The EMBO journal ·Vol. 5 ·No. 13 ·1986-12-20 ·Pages 3681-5

Kuhn A, Kreil G, Wickner W

Abstract

M13 procoat protein has two hydrophobic domains, one in the leader peptide and one which anchors the mature coat protein in the membrane. Disruption of the membrane anchor region by insertion of arginyl residues does not yield periplasmic coat protein. Instead, the rate of membrane assembly is slowed greater than 100-fold (t1/2 less than 5 s for wild-type, t1/2 greater than 10 min for mutant). The hydrophobic region of mature coat protein not only functions as a membrane anchor, but has an important role in the membrane assembly process per se.

MeSH Terms
Amino Acid Sequence Capsid/genetics,metabolism Capsid Proteins Coliphages/genetics Escherichia coli/genetics Membrane Proteins/genetics,metabolism Mutation Oligodeoxyribonucleotides Plasmids
Chemicals
Capsid Proteins Membrane Proteins Oligodeoxyribonucleotides coat protein, Bacteriophage M13
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kuhn A
Kreil G
Wickner W
References (24)
24 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-12-20
Pages
3681-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167410
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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