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PMID: 3541893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of two cysteine proteinases from paw-paw (Carica papaya).

The Biochemical journal ·Vol. 237 ·No. 1 ·1986-07-01 ·Pages 105-10

McKee RA, Adams S, Matthews JA, Smith CJ, Smith H

Abstract

Two cDNA clones for plant cysteine proteinases have been isolated from a Carica papaya (paw-paw, papaya) leaf tissue cDNA library by using a mixture of 16 synthetic oligodeoxyribonucleotides as a hybridization probe. The inserted regions are 311 and 440 base-pairs in length and have the potential to encode a region corresponding to the C-terminal region of two proteins which are homologous with the known plant cysteine proteinases and the mammalian thiol cathepsins. One of the sequences shows a high (greater than 77%) homology with the plant cysteine proteinase papain, the other is closely related to papaya chymopapain. One sequence contains all, and the other most, of the 3' untranslated region of the mRNA. The inserts were used as specific probes in Northern Blot analyses giving an estimated size for the two mRNA species of 1.45 kilobases.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular Cysteine Endopeptidases DNA Endopeptidases/genetics Isoenzymes/genetics Nucleic Acid Hybridization Plants/enzymology,genetics RNA, Messenger/genetics

Chemicals

Isoenzymes RNA, Messenger DNA Endopeptidases Cysteine Endopeptidases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

McKee R A

Adams S

Matthews J A

Smith C J

Smith H

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28 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2370105

Published

1986-07-01

Pages

105-10

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1146953

Subset

Databases

GENBANK

M24252

External Links

PubMed source DOI: 10.1042/bj2370105 PMC Full Text View journal details

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Molecular cloning of two cysteine proteinases from paw-paw (Carica papaya).

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