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PMID: 3531233 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparison of DNA probes and the Sereny test for identification of invasive Shigella and Escherichia coli strains.

Journal of clinical microbiology ·Vol. 24 ·No. 3 ·1986-09-00 ·Pages 498-500

Wood PK, Morris JG, Small PL, Sethabutr O, Toledo MR, Trabulsi L, Kaper JB

Abstract

Forty-two Shigella and 29 Escherichia coli strains were screened for invasiveness in the Sereny test and for hybridization with two recently described DNA probes for the invasiveness plasmid. Both probes produced identical results. All Sereny-positive strains hybridized with both DNA probes. Three Sereny-negative strains also hybridized with the probes, suggesting that there are strains containing the invasiveness plasmid that are not pathogenic in animal models.

MeSH Terms
Animals DNA, Bacterial/analysis Escherichia coli/genetics,isolation & purification,pathogenicity Guinea Pigs Humans Nucleic Acid Hybridization Plasmids Shigella/genetics,isolation & purification,pathogenicity Shigella boydii/genetics,isolation & purification,pathogenicity Shigella dysenteriae/genetics,isolation & purification,pathogenicity Shigella flexneri/genetics,isolation & purification,pathogenicity Shigella sonnei/genetics,isolation & purification,pathogenicity Virulence
Chemicals
DNA, Bacterial
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wood P K
Morris J G
Small P L
Sethabutr O
Toledo M R
Trabulsi L
Kaper J B
References (18)
18 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.24.3.498-500.1986
Published
1986-09-00
Pages
498-500
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC268950
Subset
IM
Grants
PHS HHS · 223-84-2031 · United States
External Links
PubMed source DOI: 10.1128/jcm.24.3.498-500.1986 PMC Full Text View journal details
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