Home LiteratureArticle Details

PMID: 3531183 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of the gene encoding 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli.

Journal of bacteriology ·Vol. 168 ·No. 1 ·1986-10-00 ·Pages 437-9

Woisetschläger M, Högenauer G

Abstract

The cloning of the gene for Escherichia coli PL-2 2-keto-3-deoxy-D-manno-octonate 8-phosphate synthetase is reported. Positive transformants showed an increase of approximately three-fold in specific activity of the enzyme both in E. coli and in Salmonella typhimurium as host cells. A subclone containing a 1.5-kilobase PvuII fragment overproduced active enzyme. Minicell experiments that allow the detection of plasmid encoded proteins revealed an insert-coded single protein band of 34 kilodaltons.

MeSH Terms

Aldehyde-Lyases/biosynthesis,genetics Cloning, Molecular Escherichia coli/enzymology,genetics Genes Genes, Bacterial Molecular Weight Salmonella typhimurium/enzymology,genetics

Chemicals

2-dehydro-3-deoxyphosphooctonate aldolase Aldehyde-Lyases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Woisetschläger M

Högenauer G

References (11)

11 references, click to expand

Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate).
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3756-60 PMID: 4566459
R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins.
J Bacteriol. 1974 Dec;120(3):1451-63 PMID: 4612023
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Free flow electrophoresis as a tool for enrichment of mutants with temperature-dependent lethal mutations in lipid A synthesis.
Eur J Biochem. 1985 Apr 1;148(1):21-7 PMID: 3884334
Isolation of mutants conditionally blocked in the biosynthesis of the 3-deoxy-D-manno-octulosonic-acid--lipid-A part of lipopolysaccharides derived from Salmonella typhimurium.
Eur J Biochem. 1977 Jun 1;76(1):41-9 PMID: 328282
Biosynthesis of lipid A. Enzymatic incorporation of 3-deoxy-D-mannooctulosonate into a precursor of lipid A in Salmonella typhimurium.
J Biol Chem. 1978 Mar 10;253(5):1503-11 PMID: 342527
Purification and characterization of 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli.
J Bacteriol. 1980 Feb;141(2):635-44 PMID: 6988389
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
Lipid A mutants of Salmonella typhimurium. Purification and characterization of a lipid A precursor produced by a mutant in 3-deoxy-D-mannooctulosonate-8-phosphate synthetase.
J Biol Chem. 1977 Jul 25;252(14):4904-12 PMID: 17608

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.168.1.437-439.1986

Published

1986-10-00

Pages

437-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC213473

Subset

External Links

PubMed source DOI: 10.1128/jb.168.1.437-439.1986 PMC Full Text View journal details

Analysis Services

Cloning and characterization of the gene encoding 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli.

Contact

WeChat Official Account