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PMID: 352910 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-giemsa bands on human chromosomes.

Human genetics ·Vol. 43 ·No. 1 ·1978-07-12 ·Pages 53-6

Howell WM, Black DA

Abstract

A simple and rapid technique is described whereby the nucleolus organizer regions (NORs) of human chromosomes can be differentially stained with silver. This staining is followed by trypsin-Giemsa banding on the same metaphase chromosomes. The metaphases simultaneously exhibit silver-stained NORs and G bands, allowing for the unequivocal identification of all chromosomes and greatly facilitating studies involving the NOR-bearing acrocentrics.

MeSH Terms
Azure Stains Cell Nucleolus Chromosomes, Human Cytological Techniques Genes Genetic Techniques Humans Silver
Chemicals
Azure Stains Silver
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Howell W M
Black D A
References (10)
10 references, click to expand
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    Exp Cell Res. 1969 Nov;58(1):141-52 PMID: 5408613
  2. Ag-staining of nucleolus organizer regions of chromosomes after A-,C-, G-, or R-banding procedures.
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  3. Differential staining of the satellite regions of human acrocentric chromosomes.
    Experientia. 1975 Feb 15;31(2):260-2 PMID: 46207
  4. Leukocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KCl.
    Stain Technol. 1965 Nov;40(6):333-8 PMID: 5866557
  5. An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes.
    Hum Genet. 1976 Oct 28;34(2):199-206 PMID: 63440
  6. Human nucleolar organizer chromosomes: satellite associations.
    Chromosoma. 1976 Mar 31;55(1):81-4 PMID: 56257
  7. Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining.
    Chromosoma. 1975 Nov 20;53(1):37-50 PMID: 53131
  8. Combined silver staining of the nucleolus organizing regions and Giemsa banding in human chromosomes.
    Hum Genet. 1977 Jun 10;37(1):79-80 PMID: 69605
  9. Localization of heterochromatin in human chromosomes.
    Cytogenetics. 1971;10(2):81-6 PMID: 4106483
  10. A rapid banding technique for human chromosomes.
    Lancet. 1971 Oct 30;2(7731):971-2 PMID: 4107917
Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00396478
Published
1978-07-12
Pages
53-6
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00396478 View journal details
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