Mutant of Salmonella typhimurium LT2 deficient in DNA adenine methylation.

Journal of bacteriology ·Vol. 167 ·No. 1 ·1986-07-00 ·Pages 420-2

Ritchie LJ, Hall RM, Podger DM

Abstract

A mutant of Salmonella typhimurium LT2 deficient in methylation of the adenine residues in the sequence 5'-GATC-3' was isolated. The mutation (dam-1) was linked to the cysG locus, and the properties of the mutant were similar to those of Escherichia coli dam mutants. Reversion of the hisC3076 frameshift marker by 9-aminoacridine was substantially enhanced by the dam-1 mutation, implying a direct role for adenine methylation in the prevention of frameshift mutation induction.

MeSH Terms

Adenine/metabolism Aminacrine/pharmacology DNA, Bacterial/metabolism Genes, Bacterial Methylation Methyltransferases/genetics,metabolism Mutation Salmonella typhimurium/genetics,metabolism Site-Specific DNA-Methyltransferase (Adenine-Specific)

Chemicals

DNA, Bacterial Aminacrine Methyltransferases Site-Specific DNA-Methyltransferase (Adenine-Specific) Adenine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ritchie L J

Hall R M

Podger D M

References (10)

10 references, click to expand

Location of DNA methylation genes on the Escherichia coli K-12 genetic map.
Mol Gen Genet. 1973 Dec 14;127(1):47-55 PMID: 4589344
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test.
Mutat Res. 1975 Dec;31(6):347-64 PMID: 768755
Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance.
Mol Gen Genet. 1978 Jul 25;163(3):307-12 PMID: 355857
Evidence that adenine methylation influences DNA-protein interactions in Escherichia coli.
J Bacteriol. 1985 Oct;164(1):490-3 PMID: 2995323
The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
Nucleic Acids Res. 1983 Feb 11;11(3):837-51 PMID: 6300769
Methodologies for the determination of various genetic effects in permeable strains of E. coli K-12 differing in DNA repair capacity. Quantification of DNA adduct formation, experiments with organ homogenates and hepatocytes, and animal-mediated assays.
Mutat Res. 1984 Feb;125(2):153-84 PMID: 6230533
Induction of SOS functions is not required for recA+-dependent mutagenicity of 9-aminoacridine in Salmonella typhimurium strain trpE8.
Mutat Res. 1984 Mar-Apr;131(3-4):115-21 PMID: 6371507
Frameshift mutagenesis of lambda prophage by 9-aminoacridine, proflavin and ICR-191.
Mol Gen Genet. 1984;195(3):418-23 PMID: 6236349
Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063-7 PMID: 6987663

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.167.1.420-422.1986

Published

1986-07-00

Pages

420-2

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC212898

Subset

External Links

PubMed source DOI: 10.1128/jb.167.1.420-422.1986 PMC Full Text View journal details

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Mutant of Salmonella typhimurium LT2 deficient in DNA adenine methylation.

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