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PMID: 3518629 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Recovery, growth, and production of heat-stable enterotoxin by Escherichia coli after copper-induced injury.

Applied and environmental microbiology ·Vol. 51 ·No. 4 ·1986-04-00 ·Pages 738-42

Singh A, McFeters GA

Abstract

Exposure of enterotoxigenic Escherichia coli strains to a sublethal concentration (0.75 mg/liter) of copper for 3 days at 4 degrees C induced sensitivity to deoxycholate (0.1%). When placed in a complex (brain heart infusion) or a defined amino acid salt medium, the copper-injured cells recovered their tolerance to deoxycholate in 3 and 6 h, respectively, and commenced active growth. Growth and heat-stable enterotoxin production of uninjured and copper-injured cells were studied in brain heart infusion medium. A slightly altered growth curve and an initial slow rate of toxin production were observed in injured cells when compared with those corresponding uninjured controls. However, maximum heat-stable enterotoxin levels in injured cultures were comparable to those produced by uninjured cells, suggesting that the enterotoxigenic potential of copper-injured cells was fully retained.

MeSH Terms
Animals Bacterial Toxins/biosynthesis Copper/toxicity Deoxycholic Acid/metabolism Enterotoxins/biosynthesis Escherichia coli/drug effects,growth & development,metabolism Escherichia coli Proteins Mice
Chemicals
Bacterial Toxins Enterotoxins Escherichia coli Proteins heat stable toxin (E coli) Deoxycholic Acid Copper
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Singh A
McFeters G A
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32 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.51.4.738-742.1986
Published
1986-04-00
Pages
738-42
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC238957
Subset
IM
Grants
NIAID NIH HHS · AI19089 · United States
External Links
PubMed source DOI: 10.1128/aem.51.4.738-742.1986 PMC Full Text View journal details
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