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PMID: 3513172 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 5 ·1986-03-00 ·Pages 1227-31

Toulmé JJ, Krisch HM, Loreau N, Thuong NT, Hélène C

Abstract

Synthetic oligodeoxynucleotides that are covalently linked at their 3' end to an acridine derivative and are complementary to the repeated sequence UUAAAUUAAAUUAAA adjacent to the ribosome binding site of the gene 32-encoded mRNA from phage T4 have been used to regulate the synthesis of gene 32-encoded protein in vitro. These modified, synthetic oligonucleotides specifically block the translation of gene 32-encoded mRNA with a higher efficiency than the homologous unsubstituted oligonucleotides. The inhibition produced by these short "anti-messengers" is due to the formation of specific mRNA . oligodeoxynucleotide hybrids that are stabilized by the intercalation of the acridine ring in the RNA . DNA duplex.

MeSH Terms
Acridines Base Sequence Escherichia coli Genes, Viral Intercalating Agents Nucleic Acid Hybridization Oligonucleotides/pharmacology Protein Biosynthesis RNA, Double-Stranded/metabolism RNA, Messenger/metabolism RNA, Viral/genetics Ribosomes/metabolism T-Phages/genetics Transcription, Genetic
Chemicals
Acridines Intercalating Agents Oligonucleotides RNA, Double-Stranded RNA, Messenger RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Toulmé J J
Krisch H M
Loreau N
Thuong N T
Hélène C
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.5.1227
Published
1986-03-00
Pages
1227-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323048
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.5.1227 PMC Full Text View journal details
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