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PMID: 3511028 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of genes that encode a new heat-labile enterotoxin of Escherichia coli.

Journal of bacteriology ·Vol. 165 ·No. 2 ·1986-02-00 ·Pages 348-52

Pickett CL, Twiddy EM, Belisle BW, Holmes RK

Abstract

The genes for a new enterotoxin were cloned from Escherichia coli SA53. The new toxin was heat labile and activated adenylate cyclase but was not neutralized by antisera against cholera toxin or E. coli heat-labile enterotoxin. Subcloning and minicell experiments indicated that the toxin is composed of two polypeptide subunits that are encoded by two genes. The two toxin subunits exhibited mobilities on polyacrylamide gels that are similar to those of cholera toxin and E. coli heat-labile enterotoxin subunits. A 0.8-kilobase DNA probe for the new enterotoxin failed to hybridize with the cloned structural genes for E. coli heat-labile enterotoxin.

MeSH Terms
Bacterial Toxins/genetics Cloning, Molecular Enterotoxins/genetics Escherichia coli/genetics Escherichia coli Proteins Genes Genes, Bacterial Nucleic Acid Hybridization Sequence Homology, Nucleic Acid
Chemicals
Bacterial Toxins Enterotoxins Escherichia coli Proteins heat-labile enterotoxin, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pickett C L
Twiddy E M
Belisle B W
Holmes R K
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.165.2.348-352.1986
Published
1986-02-00
Pages
348-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214422
Subset
IM
Grants
NIAID NIH HHS · 5R22 AI14107 · United States
External Links
PubMed source DOI: 10.1128/jb.165.2.348-352.1986 PMC Full Text View journal details
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