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PMID: 3501841 Published · ppublish English Journal Article

The use of tetrodotoxin for the characterization of drug-enhanced dopamine release in conscious rats studied by brain dialysis.

Naunyn-Schmiedeberg's archives of pharmacology ·Vol. 336 ·No. 5 ·1987-11-00 ·Pages 502-7

Westerink BH, Tuntler J, Damsma G, Rollema H, de Vries JB

Abstract

The effect of TTX (infused during brain dialysis of the striatum and nucleus accumbens) on the in vivo release of dopamine, 3,4-dihydroxyphenylacetic acid and homovanillic acid, was investigated. In addition it was studied whether the increase in the release of dopamine, induced by various pharmacological treatments, was sensitive to TTX infusion. The following drugs were studied: haloperidol, amphetamine, haloperidol co-administered with GBR 12909, morphine and MPP+. Dialysis was carried out in the striatum, except for morphine, which was studied in the nucleus accumbens. The infusion of TTX revealed three different types of pharmacologically enhanced dopamine release in conscious rats. First, action potential dependent dopamine release (exocytosis), which was observed in untreated animals as well as in animals treated with haloperidol, haloperidol + GBR 12909, and morphine. Second, action potential independent release (carrier-mediated) was established in the case of amphetamine. Third, action potential independent DA release, probably caused by neurotoxic reactions was observed during MPP+ infusion.

MeSH Terms
1-Methyl-4-phenylpyridinium 3,4-Dihydroxyphenylacetic Acid/metabolism Amphetamine/pharmacology Animals Corpus Striatum/metabolism Dialysis Dopamine/metabolism Haloperidol/pharmacology Homovanillic Acid/metabolism Male Morphine/pharmacology Neurotoxins/pharmacology Nucleus Accumbens/metabolism Piperazines/pharmacology Pyridinium Compounds/pharmacology Rats Rats, Inbred Strains Septal Nuclei/metabolism Tetrodotoxin/administration & dosage,pharmacology
Chemicals
Neurotoxins Piperazines Pyridinium Compounds 3,4-Dihydroxyphenylacetic Acid Tetrodotoxin Morphine vanoxerine Amphetamine Haloperidol 1-Methyl-4-phenylpyridinium Dopamine Homovanillic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Westerink B H
Department of Medicinal Chemistry, State University Groningen, The Netherlands.
Tuntler J
Damsma G
Rollema H
de Vries J B
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Article Info
Journal
Naunyn-Schmiedeberg's archives of pharmacology
Abbr.
Naunyn Schmiedebergs Arch Pharmacol
ISSN
0028-1298
DOI
10.1007/BF00169306
Published
1987-11-00
Pages
502-7
Language
English
Region
Germany
NLM ID
0326264
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00169306 View journal details
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