Home LiteratureArticle Details

PMID: 3500262 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

B cell stimulatory factor 1 (interleukin 4) is sufficient for the proliferation and differentiation of lectin-stimulated cytolytic T lymphocyte precursors.

The Journal of experimental medicine ·Vol. 166 ·No. 5 ·1987-11-01 ·Pages 1464-70

Pfeifer JD, McKenzie DT, Swain SL, Dutton RW

Abstract

In this report, we demonstrate that IL-4 is sufficient to stimulate both the proliferation and differentiation of Lyt-2+, Ia- splenic CTL precursors stimulated with the mitogenic lectin Con A. The response to IL-4 and Con A was not dependent on a putative endogenous production of IL-2 within the cultures, as demonstrated by an absence of an inhibitory effect by an anti-IL-2-R blocking mAb. Our results indicate that IL-2 and IL-4 can support an equivalent proliferative response by lectin-stimulated Lyt-2+ T lymphocytes, while IL-4 is more efficacious in stimulating their differentiation into mature cytolytically active cells.

MeSH Terms

Animals Cell Differentiation Cell Division Concanavalin A/pharmacology Cytotoxicity Tests, Immunologic Hematopoietic Stem Cells/cytology Interleukin-2/physiology Interleukin-4 Interleukins/pharmacology Mice Mice, Inbred C57BL Mice, Inbred DBA Spleen/cytology T-Lymphocytes, Cytotoxic/cytology

Chemicals

Interleukin-2 Interleukins Concanavalin A Interleukin-4

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Pfeifer J D

Department of Biology, University of California, San Diego, La Jolla 92093.

McKenzie D T

Swain S L

Dutton R W

References (21)

21 references, click to expand

A rapid method for the isolation of functional thymus-derived murine lymphocytes.
Eur J Immunol. 1973 Oct;3(10):645-9 PMID: 4587740
Regulation of cytolytic T-lymphocyte generation by B-cell stimulatory factor.
Nature. 1987 Apr 23-29;326(6115):795-8 PMID: 3494950
Significance of Lyt phenotypes: Lyt2 antibodies block activities of T cells that recognize class 1 major histocompatibility complex antigens regardless of their function.
Proc Natl Acad Sci U S A. 1981 Nov;78(11):7101-5 PMID: 6975943
Identification of a T cell-derived b cell growth factor distinct from interleukin 2.
J Exp Med. 1982 Mar 1;155(3):914-23 PMID: 6977612
A differentiation factor required for the expression of cytotoxic T-cell function.
Nature. 1982 Apr 22;296(5859):754-7 PMID: 6803172
The biochemistry, biology, and role of interleukin 2 in the induction of cytotoxic T cell and antibody-forming B cell responses.
Immunol Rev. 1982;63:129-66 PMID: 7042543
Dissection of the proliferative and differentiative signals controlling murine cytotoxic T lymphocyte responses.
J Exp Med. 1982 Jun 1;155(6):1876-81 PMID: 6978927
Characterization of helper factors distinct from interleukin 2 necessary for the generation of allospecific cytolytic T lymphocytes.
J Immunol. 1983 Feb;130(2):756-62 PMID: 6184404
The role of accessory cells in polyclonal T cell activation. I. Both induction of interleukin 2 production and of interleukin 2 responsiveness by concanavalin A are accessory cell dependent.
Eur J Immunol. 1983 Jan;13(1):1-6 PMID: 6601004
A rapid, large scale purification procedure for gibbon interleukin 2.
J Immunol. 1983 Aug;131(2):810-5 PMID: 6602847
Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells.
J Exp Med. 1983 Sep 1;158(3):836-56 PMID: 6193236
The murine IL 2 receptor. II. Monoclonal anti-IL 2 receptor antibodies as specific inhibitors of T cell function in vitro.
J Immunol. 1984 Oct;133(4):1976-82 PMID: 6432904
Interleukin 2 is both necessary and sufficient for the growth and differentiation of lectin-stimulated cytolytic T lymphocyte precursors.
J Immunol. 1985 Mar;134(3):1644-52 PMID: 3871453
Antigen stimulation of cytolytic T lymphocyte precursors: minimal requirements for growth and acquisition of cytolytic activity.
Eur J Immunol. 1985 Aug;15(8):798-803 PMID: 3875492
Properties of purified T cell subsets. I. In vitro responses to class I vs. class II H-2 alloantigens.
J Exp Med. 1985 Dec 1;162(6):2068-88 PMID: 2933483
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
J Immunol. 1986 Apr 1;136(7):2348-57 PMID: 2419430
Purification to homogeneity of B cell stimulating factor. A molecule that stimulates proliferation of multiple lymphokine-dependent cell lines.
J Exp Med. 1986 Jun 1;163(6):1405-14 PMID: 3086481
T-cell and mast cell lines respond to B-cell stimulatory factor 1.
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5654-8 PMID: 3090545
B cell stimulatory factor 1 (interleukin 4) is a potent costimulant for normal resting T lymphocytes.
J Exp Med. 1987 Jan 1;165(1):157-72 PMID: 3098893
Interleukin 4 mediates autocrine growth of helper T cells after antigenic stimulation.
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9689-93 PMID: 2948185
Monoclonal antibody directed against interleukin 2. I. Inhibition of T lymphocyte mitogenesis and the in vitro differentiation of alloreactive cytolytic T cells.
J Exp Med. 1981 Sep 1;154(3):983-8 PMID: 6974222

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.166.5.1464

Published

1987-11-01

Pages

1464-70

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2189645

Subset

Grants

PHS HHS · 7198 · United States

NIAID NIH HHS · AI-08795 · United States

NIAID NIH HHS · AI-23287 · United States

External Links

PubMed source DOI: 10.1084/jem.166.5.1464 PMC Full Text View journal details

Analysis Services

B cell stimulatory factor 1 (interleukin 4) is sufficient for the proliferation and differentiation of lectin-stimulated cytolytic T lymphocyte precursors.

Contact

WeChat Official Account