Abstract
Synthesis of the cytolytic C9-related protein (C9RP) was induced by activation of resting human peripheral T lymphocytes with the anti-CD3 antibody OKT3 or interleukin 2. Comparison of cellular cytotoxicity and C9RP content at various times during activation yielded a coefficient of correlation r = 0.92. During OKT3 stimulation of peripheral mononuclear cells, maximal C9RP content and cytotoxicity were observed by day 2 or 3, with subsequent decline to baseline values by day 5, whereas during interleukin 2 stimulation, both parameters reached the maximal level at days 3-5. After fluorescence-activated cell sorting, C9RP and cytotoxicity were quantitated in CD4+, CD8+, and Leu-19+ subsets. In OKT3-activated CD8+ cells, C9RP increased to approximately 3 X 10(6) molecules per cell, with a corresponding increase in lysis of human melanoma cells mediated by anti-CD3-anti-melanoma monoclonal antibody conjugates. Interleukin 2-stimulated CD8+ cells showed similar increases, but cytotoxicity was conjugate-independent. Activated CD4+ cells showed minimal increase in C9RP content. Leu-19+ cells, which exhibit natural killer cell activity, had a high C9RP content (approximately 2.5 X 10(6) molecules per cell) before stimulation.
MeSH Terms
Antibodies, Monoclonal/immunology
Antigens, Surface/immunology
Complement C9/biosynthesis
Cytotoxicity, Immunologic
Enzyme-Linked Immunosorbent Assay
Humans
Interleukin-2/immunology
Lymphocyte Activation
Monocytes/immunology
T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal
Antigens, Surface
Complement C9
Interleukin-2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Martin D E
Zalman L S
Jung G
Müller-Eberhard H J
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