Abstract
We have used P1 transduction to create intergeneric hybrid strains of enteric bacteria by moving the genA and hut genes between Klebsiella aerogenes, Escherichia coli and Salmonella typhimurium. The use of E. coli as the recipient in such transductions permits the construction of episomes and specialized transducing phage containing non-E. coli material. The effect of host restriction modification and deoxyribonucleic acid homology on the frequency of intergeneric transduction of these loci has been examined.
MeSH Terms
Chromosomes, Bacterial
Coliphages
DNA, Bacterial/analysis
Enterobacter/analysis,enzymology,metabolism
Escherichia coli/analysis,enzymology,metabolism
Genes
Glutamate-Ammonia Ligase/biosynthesis
Histidine/metabolism
Mutation
Nucleic Acid Conformation
Plasmids
RNA Viruses
Salmonella typhimurium/analysis,enzymology,metabolism
Transduction, Genetic
Chemicals
DNA, Bacterial
Histidine
Glutamate-Ammonia Ligase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tyler B M
Goldberg R B
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18 references, click to expand
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