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PMID: 3491868 Published · ppublish English Journal Article

Cell growth cycle block of T cell hybridomas upon activation with antigen.

The Journal of experimental medicine ·Vol. 165 ·No. 1 ·1987-01-01 ·Pages 173-94

Ashwell JD, Cunningham RE, Noguchi PD, Hernandez D

Abstract

Stimulation of antigen-specific T cell hybridomas with the appropriate antigen/MHC combination, at concentrations that resulted in the secretion of the lymphokine interleukin 2, resulted in a dose-dependent decrease in both [3H]thymidine incorporation and cell growth. Flow cytometric studies demonstrated that stimulation with antigen resulted in a cell cycle block that was most evident at the G1/S border, and mixing studies revealed that bystander T cells of different antigen specificities were not affected. For at least the large majority of T cells, the G1/S cell cycle block appeared to be irreversible after 24 h of exposure to antigen. This cell cycle block may be useful as a rapid and quantitative measure of T cell hybridoma activation, as a means of selecting T cell hybridomas that have functional alterations in the reception of stimulatory signals, and may serve as a model of the induction of tolerance in immature T cells.

MeSH Terms
Animals Antigens Cell Cycle DNA Replication Histocompatibility Antigens Class II/immunology Hybridomas/cytology Immune Tolerance Interleukin-2/metabolism Lymphocyte Activation Mice Receptors, Antigen, T-Cell/physiology T-Lymphocytes/cytology,immunology
Chemicals
Antigens Histocompatibility Antigens Class II Interleukin-2 Receptors, Antigen, T-Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ashwell J D
Cunningham R E
Noguchi P D
Hernandez D
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46 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.165.1.173
Published
1987-01-01
Pages
173-94
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188264
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.165.1.173 PMC Full Text View journal details
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