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PMID: 3488551 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Perturbation of the human T-cell antigen receptor-T3 complex leads to the production of inositol tetrakisphosphate: evidence for conversion from inositol trisphosphate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 16 ·1986-08-00 ·Pages 6098-102

Stewart SJ, Prpic V, Powers FS, Bocckino SB, Isaacks RE, Exton JH

Abstract

Antibodies directed against the T-cell antigen receptor-T3 complex mimic antigen and lead to cellular changes consistent with activation. When cells of the human T-cell line Jurkat were stimulated with a monoclonal antibody directed against T3, inositol phosphates were produced. In addition to inositol trisphosphate, which is the product of phosphatidylinositol bisphosphate cleavage, a second inositol polyphosphate was formed. This compound was more polar than inositol trisphosphate but less polar than inositol pentakisphosphate. It cochromatographed with inositol tetrakisphosphate from ostrich erythrocytes. In permeabilized Jurkat cells, this compound was shown to be formed from inositol 1,4,5-trisphosphate, but only in the presence of ATP, and 32P was incorporated into it from [gamma-32P]ATP. There also was coincident formation of inositol 1,3,4-trisphosphate. We conclude that the more polar compound is inositol tetrakisphosphate, which is formed by phosphorylation of inositol 1,4,5-trisphosphate and may be the precursor of inositol 1,3,4-trisphosphate.

MeSH Terms

Cell Line Cell Membrane Permeability Humans Inositol 1,4,5-Trisphosphate Inositol Phosphates/biosynthesis,isolation & purification,metabolism Kinetics Leukemia/immunology,metabolism Receptors, Antigen, T-Cell/immunology Sugar Phosphates/biosynthesis,metabolism T-Lymphocytes/immunology,metabolism

Chemicals

Inositol Phosphates Receptors, Antigen, T-Cell Sugar Phosphates inositol-1,3,4,5-tetrakisphosphate Inositol 1,4,5-Trisphosphate

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Stewart S J

Prpic V

Powers F S

Bocckino S B

Isaacks R E

Exton J H

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29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.16.6098

Published

1986-08-00

Pages

6098-102

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC386446

Subset

Grants

NCRR NIH HHS · RR-05424 · United States

External Links

PubMed source DOI: 10.1073/pnas.83.16.6098 PMC Full Text View journal details

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Perturbation of the human T-cell antigen receptor-T3 complex leads to the production of inositol tetrakisphosphate: evidence for conversion from inositol trisphosphate.

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