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PMID: 3487783 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Three distinct signals can induce class II gene expression in a murine pre-B-cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 13 ·1986-07-00 ·Pages 4878-82

Polla BS, Poljak A, Geier SG, Nathenson SG, Ohara J, Paul WE, Glimcher LH

Abstract

Expression of class II genes of the major histocompatibility complex (MHC) has been studied in an Abelson-murine-leukemia-virus-transformed pre-B-cell line, R8, and its class II molecule (Ia)-negative variant, R8205. These variant cells contained barely detectable levels of RNA specific for all class II genes, including the nonpolymorphic invariant chain gene (Ii), and did not express cell surface Ia. Fusion of this murine Ia-negative cell line to the human Ia-positive Raji cell produced an interspecies hybridoma that expressed the murine Ia. These data are further evidence for the existence of a trans-acting factor(s) that can regulate class II gene expression. Furthermore, the T-cell-derived lymphokine B-cell-stimulatory factor 1 (BSF-1) induced expression of class II genes in the R8205 cells. Exposure of R8205 cells to an antibody that has been shown to mimic BSF-1 activity on normal B cells also resulted in expression of class II genes. These data demonstrate that three distinct signals--a lymphokine, an alloantibody binding to membrane structures, and an interspecies trans-acting factor--can induce expression of class II genes.

MeSH Terms
Abelson murine leukemia virus Animals Antibodies, Monoclonal/immunology Antigens, Ly B-Lymphocytes/physiology Cell Line Cell Transformation, Viral Gene Expression Regulation/drug effects Growth Substances/pharmacology Histocompatibility Antigens Class II/genetics Interleukin-4 Lymphokines/pharmacology Major Histocompatibility Complex Mice
Chemicals
Antibodies, Monoclonal Antigens, Ly Growth Substances Histocompatibility Antigens Class II Lymphokines Interleukin-4
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Polla B S
Poljak A
Geier S G
Nathenson S G
Ohara J
Paul W E
Glimcher L H
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.13.4878
Published
1986-07-00
Pages
4878-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323846
Subset
IM
Grants
NIAID NIH HHS · AI-07289 · United States
NIAID NIH HHS · AI-10702 · United States
NIAID NIH HHS · AI-21569 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.13.4878 PMC Full Text View journal details
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