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PMID: 348144 Published · ppublish English Journal Article

Cell yields of Escherichia coli during anaerobic growth on fumarate and molecular hydrogen.

Archives of microbiology ·Vol. 116 ·No. 3 ·1978-03-00 ·Pages 235-8

Bernhard T, Gottschalk G

Abstract

Escherichia coli was grown anaerobically on sodium fumarate and molecular hydrogen or sodium formate in continuous culture. The maximal growth yield and the maintenance coefficient were determined. In a mineral medium a Ymax(fum) value of 6.6 g dry weight per mol fumarate was found. This value increased to 7;5 when casamino acids were present in the medium. From these data and the corresponding Ymax(ATP) values it could be calculated that per mol of fumarate reduced, 0;4 mol of ATP became available for growth. In batch culture a Yfum value of 4.8 g dry weight per mol fumarate was determined.

MeSH Terms
Adenosine Triphosphate/biosynthesis Anaerobiosis Escherichia coli/growth & development,metabolism Formates/metabolism Fumarates/metabolism Hydrogen/metabolism
Chemicals
Formates Fumarates Hydrogen Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bernhard T
Gottschalk G
References (12)
12 references, click to expand
  1. A theoretical study on the amount of ATP required for synthesis of microbial cell material.
    Antonie Van Leeuwenhoek. 1973;39(3):545-65 PMID: 4148026
  2. Growth of sulphate-reducing bacteria by fumarate dismutation.
    J Gen Microbiol. 1966 Apr;43(1):101-7 PMID: 5953822
  3. Anaerobic energy-yielding reaction associated with transhydrogenation from glycerol 3-phosphate to fumarate by an Escherichia coli system.
    J Bacteriol. 1975 Dec;124(3):1282-7 PMID: 127785
  4. A continuous culture study of an ATPase-negative mutant of Escherichia coli.
    Arch Microbiol. 1977 Jun 20;113(3):185-9 PMID: 141918
  5. Fermentation of fumarate and L-malate by Clostridium formicoaceticum.
    J Bacteriol. 1978 Jan;133(1):26-32 PMID: 618841
  6. [Study of dicarboxylic acid and pyruvate metabolism in sulfate-reducing bacteria. II. Electron transport; final acceptors].
    Ann Inst Pasteur (Paris). 1970 Mar;118(3):288-301 PMID: 5430708
  7. Electron-transport phosphorylation coupled to fumarate reduction in anaerobically grown Proteus rettgeri.
    Biochim Biophys Acta. 1974 May 22;347(2):273-89 PMID: 4407318
  8. Effects of varying the carbon source limiting growth on yield and maintenance characteristics of Escherichia coli in continuous culture.
    J Bacteriol. 1975 Sep;123(3):1076-87 PMID: 169226
  9. The role of energy-spilling reactions in the growth of Klebsiella aerogenes NCTC 418 in aerobic chemostat culture.
    Arch Microbiol. 1976 Nov 2;110(23):305-11 PMID: 1015953
  10. Anaerobic growth of Escherichia coli on formate by reduction of nitrate, fumarate, and trimethylamine N-oxide.
    Z Allg Mikrobiol. 1977;17(3):235-42 PMID: 327708
  11. The maintenance energy of bacteria in growing cultures.
    Proc R Soc Lond B Biol Sci. 1965 Oct 12;163(991):224-31 PMID: 4378482
  12. H2-dependent anaerobic growth of Escherichia coli on L-malate: succinate formation.
    J Bacteriol. 1976 Feb;125(2):423-8 PMID: 1107323
Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
Published
1978-03-00
Pages
235-8
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
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