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PMID: 3476929 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human lens gamma-crystallins: isolation, identification, and characterization of the expressed gene products.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 17 ·1987-09-00 ·Pages 6088-92

Siezen RJ, Thomson JA, Kaplan ED, Benedek GB

Abstract

We have isolated the individual gamma-crystallins expressed in young human lenses and identified with which of the six known human gamma-crystallin genes they each correspond. We find that at least 90% of the gamma-crystallins synthesized in the young human lens are the products of genes gamma G3 and gamma G4. We demonstrate that gamma G4-crystallin undergoes a temperature-dependent phase separation, and we have measured the low-concentration branch of its coexistence curve (phase separation temperature vs. concentration) up to about 40 mg/ml. By comparison, we found no evidence of gamma G3-crystallin phase separating, even at lower temperatures and higher concentrations. This is consistent with predictions based on sequence homology between human and rat gamma-crystallins. The implications of these findings for human inherited and senile cataracts are considered.

MeSH Terms
Aging/metabolism Amino Acids/analysis Animals Cataract/etiology Cattle Child, Preschool Crystallins/genetics,isolation & purification Humans Infant Multigene Family Rats
Chemicals
Amino Acids Crystallins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Siezen R J
Thomson J A
Kaplan E D
Benedek G B
References (42)
42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.17.6088
Published
1987-09-00
Pages
6088-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299013
Subset
IM
Grants
NEI NIH HHS · EY05127 · United States
NEI NIH HHS · EY05496 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.17.6088 PMC Full Text View journal details
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