Home LiteratureArticle Details
PMID: 3473275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Separation of temporal control and trans-acting modulation of flagellin and chemotaxis genes in Caulobacter.

Molecular & general genetics : MGG ·Vol. 206 ·No. 2 ·1987-02-00 ·Pages 300-6

Bryan R, Champer R, Gomes S, Ely B, Shapiro L

Abstract

The genes involved in the biogenesis of the flagellum and the chemotaxis machinery are temporally regulated during the Caulobacter crescentus cell cycle. Using plasmid complementation, we have mapped the extent of the flaY and flaE genes. These genes function in trans to regulate the expression of the flagellin genes and the chemotaxis genes. We have found that the trans regulation that modulates the amount of the flagellins and the chemotaxis proteins can be separated from the temporal control of fla and che gene expression. This conclusion is based on two observations: the low level of synthesis of flagellins and chemotaxis proteins in flaY and flaE mutant strains occurred at the correct time in the cell cycle, and complementation with plasmids containing intact flaY and flaE genes resulted in the synthesis of normal levels of flagellins and chemotaxis gene products with the maintenance of temporal cell cycle control.

MeSH Terms
Bacteria/genetics Bacterial Physiological Phenomena Bacterial Proteins/genetics Carboxylic Ester Hydrolases/genetics,metabolism Chemotactic Factors/genetics Chemotaxis Flagellin/genetics Genes Genes, Bacterial Membrane Proteins/genetics,metabolism Methyl-Accepting Chemotaxis Proteins Methyltransferases/genetics,metabolism Plasmids
Chemicals
Bacterial Proteins Chemotactic Factors Membrane Proteins Methyl-Accepting Chemotaxis Proteins Flagellin Methyltransferases Carboxylic Ester Hydrolases chemotactic protein methylesterase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bryan R
Champer R
Gomes S
Ely B
Shapiro L
References (25)
25 references, click to expand
  1. Envelope-associated nucleoid from Caulobacter crescentus stalked and swarmer cells.
    J Bacteriol. 1977 Oct;132(1):294-301 PMID: 334726
  2. The nucleotide sequence of the Mr = 28,500 flagellin gene of Caulobacter crescentus.
    J Biol Chem. 1983 Jun 25;258(12):7395-401 PMID: 6305939
  3. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  4. Analysis of nonmotile mutants of the dimorphic bacterium Caulobacter crescentus.
    J Bacteriol. 1979 Jan;137(1):627-34 PMID: 762024
  5. Identification of a protein methyltransferase as the cheR gene product in the bacterial sensing system.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):533-7 PMID: 322131
  6. Analysis of the pleiotropic regulation of flagellar and chemotaxis gene expression in Caulobacter crescentus by using plasmid complementation.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1341-5 PMID: 6324186
  7. Synthesis and assembly of flagellar components by Caulobacter crescentus motility mutants.
    J Bacteriol. 1983 Jun;154(3):1137-44 PMID: 6853442
  8. Flagellar hook and basal complex of Caulobacter crescentus.
    J Bacteriol. 1979 Jun;138(3):984-9 PMID: 457596
  9. Genetic mapping of genes required for motility in Caulobacter crescentus.
    Genetics. 1984 Nov;108(3):523-32 PMID: 6437899
  10. Cascade regulation of Caulobacter flagellar and chemotaxis genes.
    J Mol Biol. 1987 Mar 5;194(1):71-80 PMID: 3039148
  11. Methylation involved in chemotaxis is regulated during Caulobacter differentiation.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5261-5 PMID: 6577421
  12. Fusions of flagellar operons to lactose genes on a mu lac bacteriophage.
    J Bacteriol. 1982 Apr;150(1):16-26 PMID: 7037746
  13. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  14. Generation of polarity during Caulobacter cell differentiation.
    Annu Rev Cell Biol. 1985;1:173-207 PMID: 2881560
  15. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  16. Synthesis of specific membrane proteins is a function of DNA replication an phospholipid synthesis in Caulobacter crescentus.
    J Mol Biol. 1982 Aug 5;159(2):303-22 PMID: 7143443
  17. Temporal and spatial control of flagellar and chemotaxis gene expression during Caulobacter cell differentiation.
    Cold Spring Harb Symp Quant Biol. 1985;50:831-40 PMID: 3938370
  18. Physical mapping and complementation analysis of transposon Tn5 mutations in Caulobacter crescentus: organization of transcriptional units in the hook gene cluster.
    J Bacteriol. 1984 Jun;158(3):897-904 PMID: 6327646
  19. Differential expression and positioning of chemotaxis methylation proteins in Caulobacter.
    J Mol Biol. 1984 Sep 25;178(3):551-68 PMID: 6492158
  20. Transfer of drug resistance factors to the dimorphic bacterium Caulobacter crescentus.
    Genetics. 1979 Mar;91(3):371-80 PMID: 378764
  21. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP.
    Bacteriol Rev. 1964 Sep;28:231-95 PMID: 14220656
  22. Isolation of a Caulobacter gene cluster specifying flagellum production by using nonmotile Tn5 insertion mutants.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6797-801 PMID: 16593248
  23. Differential membrane phospholipid synthesis during the cell cycle of Caulobacter crescentus.
    J Bacteriol. 1980 Jan;141(1):262-9 PMID: 7353999
  24. Methylation of a membrane protein involved in bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3939-43 PMID: 1105570
  25. Generalized Transduction in CAULOBACTER CRESCENTUS.
    Genetics. 1977 Nov;87(3):391-9 PMID: 17248770
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333588
Published
1987-02-00
Pages
300-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 11301 · United States
NIGMS NIH HHS · GM 32506 · United States
NIGMS NIH HHS · GM 33580 · United States
External Links
PubMed source DOI: 10.1007/BF00333588
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2