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PMID: 3470759 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Continuing rearrangement of immunoglobulin and T-cell receptor genes in a Ha-ras-transformed lymphoid progenitor cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 7 ·1987-04-00 ·Pages 1799-803

Alessandrini A, Pierce JH, Baltimore D, Desiderio SV

Abstract

The arrangement of immunoglobulin genes has been examined in a series of lymphoid cell lines transformed with the Harvey murine sarcoma virus. One cell line, HAFTL-1, expresses antigenic markers characteristic of B-lymphoid cells and undergoes frequent rearrangement at the JH locus (where J = joining and H = heavy chain) during propagation in culture. By molecular cloning and nucleotide sequence determination, these rearrangements were found to represent the earliest postulated step in heavy chain gene assembly: the joining of a diversity (D) segment to a JH segment. The HAFTL-1 cell line also undergoes infrequent D beta-to-J beta joining at the T-cell receptor beta locus in culture. The observations presented here suggest that the HAFTL-1 cell line represents the early stage of B-cell differentiation at which immunoglobulin gene rearrangement is initiated.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Neoplastic Cloning, Molecular DNA/isolation & purification Genes Harvey murine sarcoma virus/genetics Immunoglobulins/genetics Mice Oncogenes Receptors, Antigen, T-Cell/genetics
Chemicals
Immunoglobulins Receptors, Antigen, T-Cell DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Alessandrini A
Pierce J H
Baltimore D
Desiderio S V
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.7.1799
Published
1987-04-00
Pages
1799-803
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304528
Subset
IM
Grants
NCI NIH HHS · 2 T32 CA09139 · United States
NCI NIH HHS · 5 P01 CA16519 · United States
Databases
GENBANK
M15803
External Links
PubMed source DOI: 10.1073/pnas.84.7.1799 PMC Full Text View journal details
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