Abstract
In vertebrate retinal rod outer segments, transducin, a guanine-nucleotide-binding protein, mediates signal coupling between rhodopsin and cyclic GMP phosphodiesterase. Whereas the T alpha subunit (39 kDa) of transducin binds guanine nucleotides and is the activator of the phosphodiesterase, the T beta gamma subunits (35 and 10 kDa) may function to physically link T alpha with photolysed rhodopsin. We have previously reported that a site of binding of transducin is on the C-terminus of bovine rhodopsin. By using competition with synthetic peptides, the recognition region was localized to bovine opsin amino acid residues 317-339. Further studies are detailed which determine the boundaries of this binding site on rhodopsin, as well as some of the critical amino acids needed for transducin binding. These results suggest that the serine and threonine residues in the rhodopsin C-terminal peptides Rhod-1 and Rhod-3 are critical for reconstitution of transducin GTPase activity.
MeSH Terms
Amino Acid Sequence
Animals
Binding Sites
Cattle
GTP Phosphohydrolases/metabolism
Membrane Proteins/metabolism
Peptides/chemical synthesis,metabolism
Retinal Pigments/metabolism
Rhodopsin/metabolism
Rod Cell Outer Segment/metabolism
Structure-Activity Relationship
Transducin
Chemicals
Membrane Proteins
Peptides
Retinal Pigments
Rhodopsin
GTP Phosphohydrolases
Transducin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takemoto D J
Morrison D
Davis L C
Takemoto L J
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