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PMID: 3460062 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of processing events in the synthesis of platelet-derived growth factor-like proteins by human osteosarcoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 13 ·1986-07-00 ·Pages 4636-40

Graves DT, Owen AJ, Williams SR, Antoniades HN

Abstract

The human osteosarcoma-derived cell line U-2 OS expresses c-sis mRNA and synthesizes platelet-derived growth factor (PDGF)-like proteins. Pulse-chase experiments indicate that proteins of 23 kDa and 180 kDa are synthesized first. The 23-kDa protein undergoes dimerization and proteolysis, giving rise to the 30-kDa dimeric protein secreted by the cells. The 180-kDa protein is proteolytically cleaved in a complex series of steps that give rise to several intracellular species. It is also the likely precursor of high molecular mass PDGF-like or PDGF-associated proteins secreted by these cells. The processing and secretion of the 180-kDa protein is slower than that of the 23-kDa protein. Subcellular fractionation and studies with the antibiotic monensin indicate that the processing events occur in the Golgi-endoplasmic reticulum compartment of U-2 OS cells.

MeSH Terms
Cell Line Chemical Precipitation Endoplasmic Reticulum/metabolism Golgi Apparatus/drug effects Humans Kinetics Molecular Weight Monensin/pharmacology Osteosarcoma/metabolism Platelet-Derived Growth Factor/biosynthesis,immunology Protein Processing, Post-Translational
Chemicals
Platelet-Derived Growth Factor Monensin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Graves D T
Owen A J
Williams S R
Antoniades H N
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.13.4636
Published
1986-07-00
Pages
4636-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323796
Subset
IM
Grants
NCI NIH HHS · CA30101 · United States
NCI NIH HHS · CA40214 · United States
NIDCR NIH HHS · DE07006 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.13.4636 PMC Full Text View journal details
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