Home LiteratureArticle Details
PMID: 3437886 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and characterization of chicken ovalbumin gene upstream promoter transcription factor from homologous oviduct cells.

Molecular and cellular biology ·Vol. 7 ·No. 12 ·1987-12-00 ·Pages 4151-8

Bagchi MK, Tsai SY, Tsai MJ, O'Malley BW

Abstract

Previous studies established that the chicken ovalbumin gene upstream promoter (COUP) sequence, which lies between -70 and -90 base pairs upstream from the cap site, is essential for the efficient transcription of the ovalbumin gene. A transcription factor which binds to this sequence has been purified from the homologous chicken oviduct cells. The purification scheme starting from oviduct nuclear extract involved a combination of conventional column and sequence-specific DNA affinity chromatography steps. Using gel retardation and DNase I footprinting techniques to assay COUP-binding activity, we achieved extensive purification of this factor. Binding competition studies with the purified factor indicated that it bound specifically to the COUP sequence and that the binding could be competed for only by the promoter DNA fragments or synthetic oligonucleotides containing the COUP sequence. The purified protein preparation showed multiple polypeptide bands on polyacrylamide gel electrophoresis. Renaturation of separated polypeptides after extraction from the gel matrix was carried out. The majority of renatured polypeptides exhibited specific binding to the COUP sequence.

MeSH Terms
Animals Binding, Competitive Cell Nucleus/analysis Chickens Chromatography Chromatography, Affinity DNA/metabolism Deoxyribonuclease I Electrophoresis, Polyacrylamide Gel Female Molecular Weight Ovalbumin/genetics Oviducts/analysis Promoter Regions, Genetic Protein Denaturation Transcription Factors/isolation & purification,metabolism Transcription, Genetic
Chemicals
Transcription Factors Ovalbumin DNA Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bagchi M K
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Tsai S Y
Tsai M J
O'Malley B W
References (39)
39 references, click to expand
  1. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
    Nucleic Acids Res. 1981 Jul 10;9(13):3047-60 PMID: 6269071
  2. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
    Anal Biochem. 1980 Nov 15;109(1):76-86 PMID: 6258458
  3. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
    Anal Biochem. 1981 Nov 1;117(2):307-10 PMID: 6172996
  4. Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
    Cell. 1983 Mar;32(3):669-80 PMID: 6187469
  5. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  6. Definition of the ovalbumin gene promoter by transfer of an ovalglobin fusion gene into cultured cells.
    Nucleic Acids Res. 1983 Oct 11;11(19):6733-54 PMID: 6314256
  7. A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity.
    Cell. 1984 Feb;36(2):357-69 PMID: 6537904
  8. Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.
    Nature. 1984 May 17-23;309(5965):229-34 PMID: 6325944
  9. A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.
    Cell. 1984 May;37(1):273-83 PMID: 6722872
  10. Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin.
    Nature. 1984 Sep 6-11;311(5981):81-4 PMID: 6472466
  11. Multiple specific contacts between a mammalian transcription factor and its cognate promoters.
    Nature. 1984 Nov 29-Dec 5;312(5993):409-13 PMID: 6095100
  12. Identification by exonuclease footprinting of a distal promoter-binding protein from HeLa cell extracts.
    DNA. 1985 Jun;4(3):233-40 PMID: 2988892
  13. Sequences required for in vitro transcriptional activation of a Drosophila hsp 70 gene.
    Cell. 1985 Sep;42(2):527-37 PMID: 4028160
  14. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
    Cell. 1985 Sep;42(2):559-72 PMID: 2992804
  15. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins.
    Nature. 1985 Aug 29-Sep 4;316(6031):774-8 PMID: 4041012
  16. Sp1 binds to promoter sequences and activates herpes simplex virus 'immediate-early' gene transcription in vitro.
    Nature. 1985 Sep 12-18;317(6033):179-82 PMID: 2993923
  17. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
    Cell. 1985 Nov;43(1):165-75 PMID: 4075392
  18. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
    Cell. 1985 Dec;43(2 Pt 1):439-48 PMID: 4075400
  19. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  20. Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene.
    Cell. 1986 Feb 28;44(4):565-76 PMID: 3004739
  21. Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter.
    EMBO J. 1985 Dec 16;4(13A):3563-70 PMID: 4092688
  22. Binding of Drosophila heat-shock gene transcription factor to the hsp 70 promoter. Evidence for symmetric and dynamic interactions.
    J Biol Chem. 1986 Jun 15;261(17):7934-40 PMID: 3711119
  23. Affinity purification of sequence-specific DNA binding proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5889-93 PMID: 3461465
  24. Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.
    Science. 1986 Oct 3;234(4772):47-52 PMID: 3529394
  25. Partial purification of a nuclear protein that binds to the CCAAT box of the mouse alpha 1-globin gene.
    Mol Cell Biol. 1986 Mar;6(3):821-32 PMID: 3464831
  26. Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene.
    Mol Cell Biol. 1986 Aug;6(8):2784-91 PMID: 3785213
  27. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  28. Identification of two factors required for transcription of the ovalbumin gene.
    Mol Cell Biol. 1986 Dec;6(12):4259-67 PMID: 3796602
  29. A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
    Mol Cell Biol. 1986 Dec;6(12):4723-33 PMID: 3796614
  30. The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties.
    Cell. 1987 Feb 13;48(3):507-15 PMID: 3100052
  31. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE.
    J Biol Chem. 1987 Mar 5;262(7):3310-21 PMID: 3029109
  32. Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site.
    J Biol Chem. 1987 Mar 5;262(7):3322-30 PMID: 3818643
  33. Promoters, activator proteins, and the mechanism of transcriptional initiation in yeast.
    Cell. 1987 May 8;49(3):295-7 PMID: 2882858
  34. Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography.
    Eur J Biochem. 1975 Jun;54(2):411-8 PMID: 1100376
  35. A significant lag in the induction of ovalbumin messenger RNA by steroid hormones: a receptor translocation hypothesis.
    Cell. 1976 Aug;8(4):557-72 PMID: 182385
  36. Transcription of structural and intervening sequences in the ovalbumin gene and identification of potential ovalbumin mRNA precursors.
    Cell. 1978 Oct;15(2):671-85 PMID: 719758
  37. Effect of estrogen on gene expression in chicken oviduct: evidence for transcriptional control of ovalbumin gene.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1049-53 PMID: 286292
  38. The ovalbumin gene: organization, structure, transcription, and regulation.
    Recent Prog Horm Res. 1979;35:1-46 PMID: 229523
  39. Transcription factors from oviduct and HeLa cells are similar.
    J Biol Chem. 1981 Dec 25;256(24):13055-9 PMID: 6895517
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.12.4151-4158.1987
Published
1987-12-00
Pages
4151-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368095
Subset
IM
Grants
NICHD NIH HHS · HD-08188 · United States
NICHD NIH HHS · HD-17379 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.12.4151-4158.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2