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PMID: 3426400 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Variations in glycosylation of the influenza virus hemagglutinin of subtype H7. Brief report.

Archives of virology ·Vol. 97 ·No. 3-4 ·1987-00-00 ·Pages 359-63

Klenk HD, Schwarz RT

Abstract

The numbers of the carbohydrate side chains have been analyzed on a series of subtype 7 hemagglutinins of influenza A virus. The procedure employed involves growth of the virus in the presence of the trimming inhibitor N-methyl-1-desoxynojirimycin followed by controlled removal of the resulting mannose-rich oligosaccharides with endo-N-acetylglucosaminidase H. By comparative analysis with virus grown in the absence of the inhibitor oligomannosidic side chains can be discriminated from complex ones. The results show that all H7 hemagglutinins have a complex and an oligomannosidic side chain on HA2, whereas the oligosaccharides of HA1 vary in number.

MeSH Terms

Animals Genetic Variation Glycosylation Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/genetics Humans Influenza A virus/classification,genetics Oligosaccharides/analysis Species Specificity

Chemicals

Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral Oligosaccharides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Klenk H D

Institut für Virologie, Justus-Liebig-Universität, Giessen, Federal Republic of Germany.

Schwarz R T

References (10)

10 references, click to expand

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Article Info

Journal

Archives of virology

Abbr.

Arch Virol

ISSN

0304-8608

DOI

10.1007/BF01314433

Published

1987-00-00

Pages

359-63

Language

English

Region

Austria

NLM ID

7506870

Subset

External Links

PubMed source DOI: 10.1007/BF01314433 View journal details

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Variations in glycosylation of the influenza virus hemagglutinin of subtype H7. Brief report.

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