Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.

Applied and environmental microbiology ·Vol. 53 ·No. 10 ·1987-10-00 ·Pages 2588-9

Lippke JA, Strzempko MN, Raia FF, Simon SL, French CK

Abstract

A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.

MeSH Terms

Bacteroides/genetics DNA, Bacterial/isolation & purification Electrophoresis, Agar Gel Guanidines Humans Isothiocyanates Thiocyanates Ultracentrifugation

Chemicals

DNA, Bacterial Guanidines Isothiocyanates Thiocyanates guanidine isothiocyanate guanidine thiocyanate

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lippke J A

BioTechnica Diagnostics, Cambridge, Massachusetts 02138.

Strzempko M N

Raia F F

Simon S L

French C K

References (1)

1 references, click to expand

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.53.10.2588-2589.1987

Published

1987-10-00

Pages

2588-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC204150

Subset

External Links

PubMed source DOI: 10.1128/aem.53.10.2588-2589.1987 PMC Full Text View journal details

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Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.

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