Functional role of a highly repetitive DNA sequence in anchorage of the mouse genome.

Nucleic acids research ·Vol. 16 ·No. 17 ·1988-09-12 ·Pages 8351-60

Neuer-Nitsche B, Lu XN, Werner D

Abstract

The major portion of the eukaryotic genome consists of various categories of repetitive DNA sequences which have been studied with respect to their base compositions, organizations, copy numbers, transcription and species specificities; their biological roles, however, are still unclear. A novel quality of a highly repetitive mouse DNA sequence is described which points to a functional role: All copies (approximately 50,000 per haploid genome) of this DNA sequence reside on genomic Alu I DNA fragments each associated with nuclear polypeptides that are not released from DNA by proteinase K, SDS and phenol extraction. By this quality the repetitive DNA sequence is classified as a member of the sub-set of DNA sequences involved in tight DNA-polypeptide complexes which have been previously shown to be components of the subnuclear structure termed 'nuclear matrix'. From these results it has to be concluded that the repetitive DNA sequence characterized in this report represents or comprises a signal for a large number of site specific attachment points of the mouse genome in the nuclear matrix.

MeSH Terms

Animals Base Sequence Carcinoma, Ehrlich Tumor/genetics DNA, Neoplasm/genetics,isolation & purification Mice Molecular Sequence Data Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid

Chemicals

DNA, Neoplasm

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Neuer-Nitsche B

Institut für Zell- und Tumorbiologie, Deutsches Krebsforschungszentrum, Heidelberg, FRG.

Lu X N

Werner D

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.17.8351

Published

1988-09-12

Pages

8351-60

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338563

Subset

External Links

PubMed source DOI: 10.1093/nar/16.17.8351 PMC Full Text View journal details

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Functional role of a highly repetitive DNA sequence in anchorage of the mouse genome.

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