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PMID: 3413097 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Intramolecular DNA triplexes in supercoiled plasmids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 17 ·1988-09-00 ·Pages 6292-6

Hanvey JC, Shimizu M, Wells RD

Abstract

A series of inserts with oligopurine.oligopyrimidine mirror repeat sequences was investigated at the base pair level with specific chemical probes (OsO4 and diethylpyrocarbonate) to evaluate the in vitro existence of intramolecular triplexes. Two parent inserts in recombinant plasmids with (GAA)9 and (AG)12 sequences and three mutant inserts (containing transitions or transversions) revealed that base pair changes at one location affected the chemical reactivity 13 base pairs away. The specificity and nature of these reactions, as well as the thermal stability of the complexes, provide direct evidence for the existence of a triplex with a portion of the pyrimidine-rich strand folded back and Hoogsteen-paired in the major groove of the Watson-Crick duplex. The biological implications of this unorthodox DNA structure are discussed.

MeSH Terms
Base Sequence DNA, Superhelical Macromolecular Substances Molecular Sequence Data Nucleic Acid Conformation Plasmids Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Superhelical Macromolecular Substances
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hanvey J C
Department of Biochemistry, School of Medicine, University of Alabama, Birmingham 35294.
Shimizu M
Wells R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.17.6292
Published
1988-09-00
Pages
6292-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281955
Subset
IM
Grants
NIGMS NIH HHS · GM 30822 · United States
NIAID NIH HHS · T32 AI07150 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.17.6292 PMC Full Text View journal details
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