Detection and subcellular localization of two Sym plasmid-dependent proteins of Rhizobium leguminosarum biovar viciae.

Journal of bacteriology ·Vol. 170 ·No. 9 ·1988-09-00 ·Pages 4424-7

de Maagd RA, Wijffelman CA, Pees E, Lugtenberg BJ

Abstract

The previously described Sym plasmid-dependent 24-kilodalton rhi protein of Rhizobium leguminosarum biovar viciae was localized in the cytosol fraction. Another Sym plasmid-dependent protein of 50 kilodaltons is secreted into the growth medium, and its expression is dependent on both the nodD gene and a nod gene inducer.

MeSH Terms

Bacterial Proteins/analysis,genetics Cytosol/analysis Flavanones Flavonoids/pharmacology Gene Expression Regulation Genes, Bacterial/drug effects Immunoassay Lipopolysaccharides/analysis,genetics Plasmids Rhizobium/analysis,drug effects,genetics,ultrastructure Symbiosis

Chemicals

Bacterial Proteins Flavanones Flavonoids Lipopolysaccharides naringenin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

de Maagd R A

Department of Plant Molecular Biology, Leiden University, The Netherlands.

Wijffelman C A

Pees E

Lugtenberg B J

References (14)

14 references, click to expand

Induction of the nodA promoter of Rhizobium leguminosarum Sym plasmid pRL1JI by plant flavanones and flavones.
J Bacteriol. 1987 Jan;169(1):198-204 PMID: 3539915
A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes.
Science. 1986 Aug 29;233(4767):977-80 PMID: 3738520
The nodD gene of Rhizobium leguminosarum is autoregulatory and in the presence of plant exudate induces the nodA,B,C genes.
EMBO J. 1985 Dec 16;4(13A):3369-73 PMID: 16453650
The Isolation and Partial Characterization of the Lipopolysaccharides from Several Rhizobium trifolii Mutants Affected in Root Hair Infection.
Plant Physiol. 1987 Jun;84(2):421-7 PMID: 16665455
Recognition of individual strains of fast-growing rhizobia by using profiles of membrane proteins and lipopolysaccharides.
J Bacteriol. 1988 Aug;170(8):3782-5 PMID: 3403513
Fractionation of Rhizobium leguminosarum cells into outer membrane, cytoplasmic membrane, periplasmic, and cytoplasmic components.
J Bacteriol. 1986 Sep;167(3):1083-5 PMID: 3745116
The Rhizobium leguminosarum nodulation gene nodF encodes a polypeptide similar to acyl-carrier protein and is regulated by nodD plus a factor in pea root exudate.
EMBO J. 1986 Apr;5(4):647-52 PMID: 16453679
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
Expression of the nodulation gene nod C of Rhizobium meliloti in Escherichia coli: role of the nod C gene product in nodulation.
EMBO J. 1985 Oct;4(10):2425-30 PMID: 15929218
Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
R factor transfer in Rhizobium leguminosarum.
J Gen Microbiol. 1974 Sep;84(1):188-98 PMID: 4612098
Expression of the nodulation gene nodA in Rhizobium meliloti and localization of the gene product in the cytosol.
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9581-5 PMID: 16593791
Identification of a rhizosphere protein encoded by the symbiotic plasmid of Rhizobium leguminosarum.
J Bacteriol. 1984 May;158(2):621-7 PMID: 6327615
A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.170.9.4424-4427.1988

Published

1988-09-00

Pages

4424-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC211466

Subset

External Links

PubMed source DOI: 10.1128/jb.170.9.4424-4427.1988 PMC Full Text View journal details

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Detection and subcellular localization of two Sym plasmid-dependent proteins of Rhizobium leguminosarum biovar viciae.

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