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PMID: 338600 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages.

Journal of bacteriology ·Vol. 133 ·No. 1 ·1978-01-00 ·Pages 91-100

Fletcher G, Irwin CA, Henson JM, Fillingim C, Malone MM, Walker JR

Abstract

From a lysogen with lambda integrated in the leu operon, specialized transducing phages that carry the cell division, murein biosynthesis, and envelope permeability genes located about 0.5 min to the right of leu were isolated. These phages were used to identify the previously undiscovered cell division gene sep. A genetic map proves that sep is located in the sequence leuA sep murE murF murC ddl ftsA envA. A physical map of this region was prepared by heteroduplex analysis of the phage DNAs. Overlapping segments of host DNA extended rightward for as much as 26.4 kilobase pairs from the prophage insertion point (thought to be in leuA) to include all the genes through envA.

MeSH Terms
Cell Division Chromosome Mapping Coliphages Escherichia coli/genetics Genes Genetic Linkage Leucine/metabolism Microscopy, Electron Operon Transduction, Genetic
Chemicals
Leucine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fletcher G
Irwin C A
Henson J M
Fillingim C
Malone M M
Walker J R
References (21)
21 references, click to expand
  1. Small polydisperse circular DNA of HeLa cells.
    J Mol Biol. 1972 Aug 21;69(2):163-78 PMID: 5070865
  2. Cell division in a chain-forming envA mutant of Escherichia coli K12. Fine structure of division sites and effects of EDTA, lysozyme and ampicillin.
    Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):651-64 PMID: 4999789
  3. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
    Virology. 1970 Mar;40(3):734-44 PMID: 4908735
  4. Genetics of a chain-forming mutant of Escherichia coli. Transduction and dominance of the envA gene mediating increased penetration to some antibacterial agents.
    Genet Res. 1970 Aug;16(1):63-78 PMID: 4922970
  5. Replication of coliphage M-13. II. Intracellular deoxyribonucleic acid forms associated with M-13 infection of mitomycin C-treated cells.
    J Virol. 1968 Nov;2(11):1296-307 PMID: 4883017
  6. The relation of the genes envA and ftsA in Escherichia coli.
    Mol Gen Genet. 1976 Aug 10;147(1):99-102 PMID: 785231
  7. Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation.
    J Bacteriol. 1975 Aug;123(2):693-703 PMID: 1097423
  8. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  9. Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme.
    J Bacteriol. 1972 Apr;110(1):35-40 PMID: 4552998
  10. Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diaminopimelic acid adding enzyme.
    J Bacteriol. 1972 Apr;110(1):41-6 PMID: 4553000
  11. Mutants of Escherichia coli with temperature-sensitive lesions in membrane phospholipid synthesis: genetic analysis of glycerol-3-phosphate acyltransferase mutants.
    Mol Gen Genet. 1972;116(3):199-210 PMID: 4558645
  12. Regulation of bacterial cell division: genetic and phenotypic analysis of temperature-sensitive, multinucleate, filament-forming mutants of Escherichia.
    J Bacteriol. 1974 Mar;117(3):978-86 PMID: 4591963
  13. Genetic analysis of bacteriophage lambda strains which transduce the genes for leucine biosynthesis.
    Mol Gen Genet. 1973 Oct 16;126(1):1-6 PMID: 4591366
  14. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  15. Temperature-sensitive mutant of Escherichia coli K-12 with an impaired D-alanine:D-alanine ligase.
    J Bacteriol. 1973 Jan;113(1):96-104 PMID: 4567144
  16. Asymmetric distribution of the transcribing regions on the complementary strands of coliphage lambda DNA.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1618-25 PMID: 5231395
  17. Formation of the parental replicative form of bacteriophage phiX174.
    J Mol Biol. 1976 Sep 15;106(2):375-402 PMID: 1086367
  18. Isolation and characterization of lambda pleu bacteriophages.
    J Bacteriol. 1977 Feb;129(2):1078-90 PMID: 320178
  19. THE SELECTION OF MUTANTS OF ESCHERICHIA COLI WITH IMPAIRED CELL DIVISION AT ELEVATED TEMPERATURE.
    Mutat Res. 1964 Jul;106:121-8 PMID: 14237063
  20. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.
    Genetics. 1964 Feb;49:237-46 PMID: 14124942
  21. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.
    Cold Spring Harb Symp Quant Biol. 1968;33:677-93 PMID: 4892005
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.133.1.91-100.1978
Published
1978-01-00
Pages
91-100
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221981
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.133.1.91-100.1978 PMC Full Text View journal details
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